Functional role of beta domain in the Thermoanaerobacter tengcongensis glucoamylase
文献类型：期刊论文
| 作者 | Li, Zilong1,2; Wei, Pingying1,2; Cheng, Hairong3,4; He, Peng1; Wang, Qinhong5; Jiang, Ning1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 98期号:5页码:2091-2099 |
| 关键词 | Prokaryotic glucoamylase Thermoanaerobacter tengcongensis glucoamylase beta domain Activity Thermostability |
| 英文摘要 | Thermoanaerobacter tengcongensis MB4 glucoamylase (TteGA) contains a catalytic domain (CD), which is structurally similar to eukaryotic GA, and a beta domain (BD) with ambiguous function. Firstly, BD is found to be essential to TteGA activity because CD alone could not hydrolyze soluble starch. However, starch hydrolysis activity, similar to that of intact TteGA, was restored to CD in the presence of BD. Secondly, BD is found to be an important helper in the correct folding of CD because CD was mainly expressed in the inclusion bodies on its own in Escherichia coli. By contrast, intact TteGA, BD, and CD combined with BD could be expressed as soluble proteins. Additionally, BD is essential to the thermostability of TteGA because CD displayed lower thermostability compared with the intact TteGA and exhibited enhanced thermostability in the presence of BD in vitro. Truncation of TteGA or mutagenesis of the residues that participate in the interdomain interaction at its BD also led to the reduced thermostability of TteGA. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | STARCH-BINDING DOMAIN ; ASPERGILLUS-AWAMORI GLUCOAMYLASE ; SACCHAROMYCOPSIS-FIBULIGERA ; THERMOSTABLE GLUCOAMYLASE ; MOLECULAR-CLONING ; THERMOACTIVE GLUCOAMYLASE ; STRUCTURE PREDICTION ; NIGER GLUCOAMYLASE ; RHIZOPUS-ORYZAE ; X-RAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000332107700015 |
| 源URL | [http://124.16.173.210/handle/834782/1362]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Dept Ind Microbiol & Biotechnol, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zilong,Wei, Pingying,Cheng, Hairong,et al. Functional role of beta domain in the Thermoanaerobacter tengcongensis glucoamylase[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(5):2091-2099. |
| APA | Li, Zilong,Wei, Pingying,Cheng, Hairong,He, Peng,Wang, Qinhong,&Jiang, Ning.(2014).Functional role of beta domain in the Thermoanaerobacter tengcongensis glucoamylase.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(5),2091-2099. |
| MLA | Li, Zilong,et al."Functional role of beta domain in the Thermoanaerobacter tengcongensis glucoamylase".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.5(2014):2091-2099. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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