Cloning, expression and characterization of a pectate lyase from Paenibacillus sp 0602 in recombinant Escherichia coli
文献类型：期刊论文
| 作者 | Li, Xiaoman1,2; Wang, Huilin1,2; Zhou, Cheng3; Ma, Yanhe3; Li, Jian4; Song, Jiangning1,2,5,6 |
| 刊名 | BMC BIOTECHNOLOGY
 |
| 出版日期 | 2014-03-10 |
| 卷号 | 14 |
| 关键词 | Pectate lyase High-level expression Characterization Degumming |
| 英文摘要 | Background: Biotechnological applications of microbial pectate lyases (Pels) in plant fiber processing are considered as environmentally friendly. As such, they become promising substitutes for conventional chemical degumming process. Since applications of Pels in various fields are widening, it is necessary to explore new pectolytic microorganisms and enzymes for efficient and effective usage. Here, we describe the cloning, expression, characterization and application of the recombinant Pel protein from a pectolytic bacterium of the genus Paenibacillus in Escherichia coli. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ALKALINE POLYGALACTURONATE LYASE ; HIGHLY METHYLATED PECTIN ; BACILLUS SP N-16-5 ; SEQUENCE ALIGNMENT ; BIOCHEMICAL-CHARACTERIZATION ; REGULATORY ELEMENTS ; DEGRADING ENZYMES ; PICHIA-PASTORIS ; HIGH-THROUGHPUT ; RAMIE FIBER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334881100001 |
| 源URL | [http://124.16.173.210/handle/834782/1370]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 4.Monash Univ, Monash Inst Pharmaceut Sci, Parkville, Vic 3052, Australia 5.Monash Univ, Fac Med, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia 6.Monash Univ, Fac Med, ARC Ctr Excellence Struct & Funct Microbial Genom, Clayton, Vic 3800, Australia |
| 推荐引用方式 GB/T 7714 | Li, Xiaoman,Wang, Huilin,Zhou, Cheng,et al. Cloning, expression and characterization of a pectate lyase from Paenibacillus sp 0602 in recombinant Escherichia coli[J]. BMC BIOTECHNOLOGY,2014,14. |
| APA | Li, Xiaoman,Wang, Huilin,Zhou, Cheng,Ma, Yanhe,Li, Jian,&Song, Jiangning.(2014).Cloning, expression and characterization of a pectate lyase from Paenibacillus sp 0602 in recombinant Escherichia coli.BMC BIOTECHNOLOGY,14. |
| MLA | Li, Xiaoman,et al."Cloning, expression and characterization of a pectate lyase from Paenibacillus sp 0602 in recombinant Escherichia coli".BMC BIOTECHNOLOGY 14(2014). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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