Metabolic Changes in Klebsiella oxytoca in Response to Low Oxidoreduction Potential, as Revealed by Comparative Proteomic Profiling Integrated with Flux Balance Analysis
文献类型：期刊论文
| 作者 | Zhu, Yan1,2; Li, Dan1; Bao, Guanhui1,2; Wang, Shaohua1,2; Mao, Shaoming1; Song, Jiangning3,4,5; Li, Yin1; Zhang, Yanping1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 80期号:9页码:2833-2841 |
| 英文摘要 | Oxidoreduction potential (ORP) is an important physiological parameter for biochemical production in anaerobic or microaerobic processes. However, the effect of ORP on cellular physiology remains largely unknown, which hampers the design of engineering strategies targeting proteins associated with ORP response. Here we characterized the effect of altering ORP in a 1,3-propanediol producer, Klebsiella oxytoca, by comparative proteomic profiling combined with flux balance analysis. Decreasing the extracellular ORP from -150 to -240 mV retarded cell growth and enhanced 1,3-propanediol production. Comparative proteomic analysis identified 61 differentially expressed proteins, mainly involved in carbohydrate catabolism, cellular constituent biosynthesis, and reductive stress response. A hypothetical oxidoreductase (HOR) that catalyzes 1,3-propanediol production was markedly upregulated, while proteins involved in biomass precursor synthesis were downregulated. As revealed by subsequent flux balance analysis, low ORP induced a metabolic shift from glycerol oxidation to reduction and rebalancing of redox and energy metabolism. From the integrated protein expression profiles and flux distributions, we can construct a rational analytic framework that elucidates how (facultative) anaerobes respond to extracellular ORP changes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | ALKYL HYDROPEROXIDE REDUCTASE ; LACTIC-ACID BACTERIA ; ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; PYRUVATE-DEHYDROGENASE ; LEUCONOSTOC-MESENTEROIDES ; ANAEROBIC FERMENTATION ; STRESS RESPONSES ; IN-SILICO ; PNEUMONIAE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334583300022 |
| 源URL | [http://124.16.173.210/handle/834782/1418]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China 4.Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 5.Monash Univ, Fac Med, Dept Biochem & Mol Biol, Melbourne, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Zhu, Yan,Li, Dan,Bao, Guanhui,et al. Metabolic Changes in Klebsiella oxytoca in Response to Low Oxidoreduction Potential, as Revealed by Comparative Proteomic Profiling Integrated with Flux Balance Analysis[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(9):2833-2841. |
| APA | Zhu, Yan.,Li, Dan.,Bao, Guanhui.,Wang, Shaohua.,Mao, Shaoming.,...&Zhang, Yanping.(2014).Metabolic Changes in Klebsiella oxytoca in Response to Low Oxidoreduction Potential, as Revealed by Comparative Proteomic Profiling Integrated with Flux Balance Analysis.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(9),2833-2841. |
| MLA | Zhu, Yan,et al."Metabolic Changes in Klebsiella oxytoca in Response to Low Oxidoreduction Potential, as Revealed by Comparative Proteomic Profiling Integrated with Flux Balance Analysis".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.9(2014):2833-2841. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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