Extracellular Overexpression of Chitosanase from Bacillus sp TS in Escherichia coli
文献类型：期刊论文
| 作者 | Zhou, Zhanping1,2; Zhao, Shuangzhi3,4; Wang, Shouquan3,4; Li, Xiaoman1,2; Su, Li3,4; Ma, Yanhe1,2; Li, Jian5; Song, Jiangning1,2,6,7 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-04-01 |
| 卷号 | 175期号:7页码:3271-3286 |
| 关键词 | Chitosanase Chitosan oligosaccharides Recombinant enzyme Extracellular expression Escherichia coli |
| 英文摘要 | The chitosanase gene from a Bacillus sp. strain isolated from soil in East China was cloned and expressed in Escherichia coli. The gene had 1224 nucleotides and encoded a mature protein of 407 amino acid residues. The optimum pH and temperature of the purified recombinant chitosanase were 5.0 and 60 A degrees C, respectively, and the enzyme was stable below 40 A degrees C. The K (m), V (max), and specific activity of the enzyme were 1.19 mg mL(-1), 674.71 mu mol min(-1) at 50 A degrees C, and 555.3 U mg(-1), respectively. Mn2+ was an activator of the recombinant chitosanase, while Co2+ was an inhibitor. Hg2+ and Cu2+ inhibited the enzyme at 1 mM. The highest level of enzyme activity (186 U mL(-1)) was achieved in culture medium using high cell-density cultivation in a 7-L fermenter. The main products of chitosan hydrolyzed by recombinant chitosanase were (GlcN)(3-6). The chitosanases was successfully secreted to the culture media through the widely used SecB-dependent type II pathway in E. coli. The high yield of the extracellular overexpression, relevant thermostability, and effective hydrolysis of commercial grade chitosan showed that this recombinant enzyme had a great potential for industrial applications. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | HEXA-N-ACETYLCHITOHEXAOSE ; WATER-SOLUBLE CHITOSAN ; GONGRONELLA SP JG ; ANTIMICROBIAL ACTIVITY ; ACETYL CHITOHEXAOSE ; PECTATE LYASE ; PURIFICATION ; EXPRESSION ; CHITIN ; OLIGOSACCHARIDES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351767200004 |
| 源URL | [http://124.16.173.210/handle/834782/1445]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Shandong Food & Fermentat Ind Res & Design Inst, Jinan 250013, Peoples R China 4.Shandong Prov Key Lab Food & Fermentat Engn, Jinan 250013, Peoples R China 5.Monash Univ, Monash Inst Pharmaceut Sci, Drug Delivery Disposit & Dynam, Parkville, Vic 3052, Australia 6.Monash Univ, Monash Bioinformat Platform, Clayton, Vic 3800, Australia 7.Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia |
| 推荐引用方式 GB/T 7714 | Zhou, Zhanping,Zhao, Shuangzhi,Wang, Shouquan,et al. Extracellular Overexpression of Chitosanase from Bacillus sp TS in Escherichia coli[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,175(7):3271-3286. |
| APA | Zhou, Zhanping.,Zhao, Shuangzhi.,Wang, Shouquan.,Li, Xiaoman.,Su, Li.,...&Song, Jiangning.(2015).Extracellular Overexpression of Chitosanase from Bacillus sp TS in Escherichia coli.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,175(7),3271-3286. |
| MLA | Zhou, Zhanping,et al."Extracellular Overexpression of Chitosanase from Bacillus sp TS in Escherichia coli".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 175.7(2015):3271-3286. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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