Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina
文献类型：期刊论文
| 作者 | Jiang, Tong1; Chan, Hsiu-Chien1; Huang, Chun-Hsiang1; Ko, Tzu-Ping2; Huang, Ting-Yung3,4; Liu, Je-Ruei5; Guo, Rey-Ting1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2013-08-16 |
| 卷号 | 438期号:1页码:193-197 |
| 关键词 | Glycoside hydrolase Crystal structure beta-Glucanase GH131 |
| 英文摘要 | beta-Glucanases have been utilized widely in industry to treat various carbohydrate-containing materials. Recently, the Podospora anserina beta-glucanase 131A (PaGluc131A) was identified and classified to a new glycoside hydrolases GH131 family. It shows exo-beta-1,3/exo-beta-1,6 and endo-beta-1,4 glucanase activities with a broad substrate specificity for laminarin, curdlan, pachyman, lichenan, pustulan, and cellulosic derivatives. Here we report the crystal structures of the PaGluc131A catalytic domain with or without ligand (cellotriose) at 1.8 angstrom resolution. The cellotriose was clearly observed to occupy the +1 to +3 subsites in substrate binding cleft. The broadened substrate binding groove may explain the diverse substrate specificity. Based on our crystal structures, the GH131 family enzyme is likely to carry out the hydrolysis through an inverting catalytic mechanism, in which E99 and E139 are supposed to serve as the general base and general acid. (C) 2013 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SOFTWARE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323467100033 |
| 源URL | [http://124.16.173.210/handle/834782/1284]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.Genozyme Biotechnol Inc, Taipei 106, Taiwan 4.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 5.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan |
| 推荐引用方式 GB/T 7714 | Jiang, Tong,Chan, Hsiu-Chien,Huang, Chun-Hsiang,et al. Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,438(1):193-197. |
| APA | Jiang, Tong.,Chan, Hsiu-Chien.,Huang, Chun-Hsiang.,Ko, Tzu-Ping.,Huang, Ting-Yung.,...&Guo, Rey-Ting.(2013).Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,438(1),193-197. |
| MLA | Jiang, Tong,et al."Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 438.1(2013):193-197. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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