Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485
文献类型：期刊论文
| 作者 | Liu, Wenting1,2; Sun, Yu3; Ko, Tzu-Ping4; Wiegel, Juergen5; Shao, Weilan3; Lu, Fuping2; Guo, Rey-Ting1; Huang, Chun-Hsiang1 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 68页码:914-916 |
| 关键词 | thermophilic enzymes xylan ss-xylosidases |
| 英文摘要 | Xylosidases hydrolyze xylopolymers at the nonreducing end to free xylose units. The beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485 was expressed in Escherichia coli and the recombinant protein was purified and crystallized. A BLASTP search with the XylC protein sequence showed that no similar structure had previously been solved. XylC was classified as a member of the new glycoside hydrolase family GH120 according to the CAZy website (http://www.cazy.org/). Crystals belonging to the monoclinic space group P21, with unit-cell parameters a = 88.36, b = 202.20, c = 99.87 angstrom, beta = 99.04 degrees, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.2 angstrom resolution. Structure determination using MIR and MAD methods is in progress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307217700014 |
| 源URL | [http://124.16.173.210/handle/834782/1315]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China 3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China 4.Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan 5.Univ Georgia, Dept Microbiol, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Liu, Wenting,Sun, Yu,Ko, Tzu-Ping,et al. Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2012,68:914-916. |
| APA | Liu, Wenting.,Sun, Yu.,Ko, Tzu-Ping.,Wiegel, Juergen.,Shao, Weilan.,...&Huang, Chun-Hsiang.(2012).Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,68,914-916. |
| MLA | Liu, Wenting,et al."Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 68(2012):914-916. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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