Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis
文献类型：期刊论文
| 作者 | Wang, Shanshan1,2; Nie, Yao1,2; Yan, Xu1,2; Ko, Tzu-Ping3; Huang, Chun-Hsiang4; Chan, Hsiu-Chien4; Guo, Rey-Ting4; Xiao, Rong5 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2014-06-01 |
| 卷号 | 70期号:70页码:800-802 |
| 英文摘要 | The NADH-dependent (R)-carbonyl reductase from Candida parapsilosis (RCR) catalyzes the asymmetric reduction of 2-hydroxyacetophenone (HAP) to produce (R)-1-phenyl-1,2-ethanediol [(R)-PED], which is used as a versatile building block for the synthesis of pharmaceuticals and fine chemicals. To gain insight into the catalytic mechanism, the structures of complexes of RCR with ligands, including the coenzyme, are important. Here, the recombinant RCR protein was expressed and purified in Escherichia coli and was crystallized in the presence of NAD(+). The crystals, which belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 85.64, b = 106.11, c = 145.55 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.15 angstrom resolution. Initial model building indicates that RCR forms a homotetramer, consistent with previous reports of medium-chain-type alcohol dehydrogenases. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | ANTI-PRELOG STEREOSPECIFICITY ; AGROBACTERIUM-RADIOBACTER AD1 ; ALCOHOL-DEHYDROGENASE ; CARBONYL REDUCTASE ; CRYSTAL-STRUCTURES ; TERNARY COMPLEX ; (R)-1-PHENYL-1,2-ETHANEDIOL ; SOFTWARE ; SYSTEM ; SUITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337062500024 |
| 源URL | [http://124.16.173.210/handle/834782/1346]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Wuxi 214122, Peoples R China 2.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 4.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 5.Rutgers State Univ, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA |
| 推荐引用方式 GB/T 7714 | Wang, Shanshan,Nie, Yao,Yan, Xu,et al. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70(70):800-802. |
| APA | Wang, Shanshan.,Nie, Yao.,Yan, Xu.,Ko, Tzu-Ping.,Huang, Chun-Hsiang.,...&Xiao, Rong.(2014).Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70(70),800-802. |
| MLA | Wang, Shanshan,et al."Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70.70(2014):800-802. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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