Immobilization of Clostridium cellulolyticum D-Psicose 3-Epimerase on Artificial Oil Bodies
文献类型：期刊论文
| 作者 | Tseng, Chih-Wen1; Liao, Chien-Yi1; Sun, Yuanxia2; Peng, Chi-Chung3; Tzen, Jason T. C.4; Guo, Rey-Ting2; Liu, Je-Ruei1,5,6 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2014-07-16 |
| 卷号 | 62期号:28页码:6771-6776 |
| 关键词 | immobilization Clostridium cellulolyticum D-psicose 3-epimerase artificial oil body |
| 英文摘要 | The rare sugar D-psicose possesses several fundamental biological functions. D-Psicose 3-epimerase from Clostridium cellulolyticum (CC-DPEase) has considerable potential for use in D-psicose production. In this study, CC-DPEase was fused to the N terminus of oleosin, a unique structural protein of seed oil bodies and was overexpressed in Escherichia colt as a CC-DPEase oleosin fusion protein. After reconstitution into artificial oil bodies (AOBs), refolding, purification, and immobilization of the active CC-DPEase were simultaneously accomplished. Immobilization of CC-DPEase on AOB increased the optimal temperature but decreased the optimal pH of the enzyme activity. Furthermore, the AOB-immobilized CC-DPEase had a thermal stability and a bioconversion rate similar to those of the free-form enzyme and retained >50% of its initial activity after five cycles of enzyme use. Thus, AOB-immobilized CC-DPEase has potential application in the production of D-psicose at a lower cost than the free-form enzyme. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Agriculture ; Chemistry ; Food Science & Technology |
| 关键词[WOS] | ESCHERICHIA-COLI ; PURIFICATION ; EXPRESSION ; ENZYMES ; SUGARS ; H10 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339226700032 |
| 源URL | [http://124.16.173.210/handle/834782/1353]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Natl Taiwan Univ, Inst Biotechnol, Taipei 10764, Taiwan 2.Chinese Acad Sci, Inst Ind Biotechnol, Tianjin, Peoples R China 3.Natl Formosa Univ, Dept Biotechnol, Huwei Township, Taiwan 4.Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan 5.Natl Taiwan Univ, Dept Anim Sci & Technol, Taipei 10764, Taiwan 6.Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan |
| 推荐引用方式 GB/T 7714 | Tseng, Chih-Wen,Liao, Chien-Yi,Sun, Yuanxia,et al. Immobilization of Clostridium cellulolyticum D-Psicose 3-Epimerase on Artificial Oil Bodies[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(28):6771-6776. |
| APA | Tseng, Chih-Wen.,Liao, Chien-Yi.,Sun, Yuanxia.,Peng, Chi-Chung.,Tzen, Jason T. C..,...&Liu, Je-Ruei.(2014).Immobilization of Clostridium cellulolyticum D-Psicose 3-Epimerase on Artificial Oil Bodies.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(28),6771-6776. |
| MLA | Tseng, Chih-Wen,et al."Immobilization of Clostridium cellulolyticum D-Psicose 3-Epimerase on Artificial Oil Bodies".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.28(2014):6771-6776. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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