Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum
文献类型：期刊论文
| 作者 | Zhang, Lilan1,2; Zhao, Puya2; Chen, Chun-Chi2; Huang, Chun-Hsiang2; Ko, Tzu-Ping3; Zheng, Yingying2; Guo, Rey-Ting2 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 70页码:946-948 |
| 英文摘要 | beta-1,3-1,4-Glucanases catalyze the specific hydrolysis of internal beta-1,4-glycosidic bonds adjacent to the 3-O-substituted glucose residues in mixed-linked beta-glucans. The thermophilic glycoside hydrolase CtGlu16A from Clostridium thermocellum exhibits superior thermal profiles, high specific activity and broad pH adaptability. Here, the catalytic domain of CtGlu16A was expressed in Escherichia coli, purified and crystallized in the trigonal space group P3(1)21, with unit-cell parameters a = b = 74.5, c = 182.9 angstrom, by the sitting-drop vapour-diffusion method and diffracted to 1.95 angstrom resolution. The crystal contains two protein molecules in an asymmetric unit. Further structural determination and refinement are in progress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | CRYSTAL-STRUCTURES ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338923200020 |
| 源URL | [http://124.16.173.210/handle/834782/1360]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan |
| 推荐引用方式 GB/T 7714 | Zhang, Lilan,Zhao, Puya,Chen, Chun-Chi,et al. Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:946-948. |
| APA | Zhang, Lilan.,Zhao, Puya.,Chen, Chun-Chi.,Huang, Chun-Hsiang.,Ko, Tzu-Ping.,...&Guo, Rey-Ting.(2014).Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,946-948. |
| MLA | Zhang, Lilan,et al."Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):946-948. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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