Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp SN5 and improvement of its thermal performance by introducing arginines substitutions
文献类型：期刊论文
| 作者 | Bai, Wenqin1,2; Zhou, Cheng1; Xue, Yanfen1; Huang, Chun-Hsiang3; Guo, Rey-Ting3; Ma, Yanhe1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 36期号:7页码:1495-1501 |
| 关键词 | Alkaline xylanase Arginines Thermophilicity Thermostability Three-Dimensional Structure X-ray crystallography Xylanase |
| 英文摘要 | The alkaline xylanase Xyn11A-LC from the alkalophilic Bacillus sp. SN5 was expressed in E. coli, purified and crystallized. The crystal structure was determined at a resolution of 1.49 . Xyn11A-LC has the beta-jelly roll structure typical of family 11 xylanases. To improve its thermostability and thermophilicity, a mutant SB3 was constructed by introducing three arginines on the different sides of the protein surface. SB3 increased the optimum temperature by 5 A degrees C. The wild type and SB3 had the half-lives of 22 and 68 min at 65 A degrees C at pH 8.0 (Tris/HCl buffer), respectively. CD spectroscopy revealed that the melting temperature (T (m)) of the wild type and SB3 were 55.3 and 66.9 A degrees C, respectively. These results showed that the introduction of arginines enhance the thermophilicity and thermostability of Xyn11A-LC. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | TRICHODERMA-REESEI ; DISULFIDE BRIDGE ; HYDROGEN-BONDS ; STABILITY ; ENDO-1,4-BETA-XYLANASE-II ; THERMOSTABILITY ; THERMOTOLERANCE ; INCREASES ; SOFTWARE ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337139500015 |
| 源URL | [http://124.16.173.210/handle/834782/1361]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, Beijing 100101, Peoples R China 2.Shanxi Normal Univ, Sch Life Sci, Linfen 041004, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Wenqin,Zhou, Cheng,Xue, Yanfen,et al. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp SN5 and improvement of its thermal performance by introducing arginines substitutions[J]. BIOTECHNOLOGY LETTERS,2014,36(7):1495-1501. |
| APA | Bai, Wenqin,Zhou, Cheng,Xue, Yanfen,Huang, Chun-Hsiang,Guo, Rey-Ting,&Ma, Yanhe.(2014).Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp SN5 and improvement of its thermal performance by introducing arginines substitutions.BIOTECHNOLOGY LETTERS,36(7),1495-1501. |
| MLA | Bai, Wenqin,et al."Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp SN5 and improvement of its thermal performance by introducing arginines substitutions".BIOTECHNOLOGY LETTERS 36.7(2014):1495-1501. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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