Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp S9
文献类型：期刊论文
| 作者 | Lv, Pin1,2; Zhang, Lilan1,2; Luo, Huiying3; Chen, Chun-Chi2; Huang, Chun-Hsiang2; Peng, Wei1,2; Wang, Kun3; Ko, Tzu-Ping4; Zheng, Yingying2; Zhang, Juankun1 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2014 |
| 卷号 | 70页码:105-107 |
| 英文摘要 | Xylanase, which catalyzes the random hydrolysis of internal xylosidic linkages, is a critical enzyme participating in xylan decomposition and has been widely applied in industrial utilizations. Xylanase isolated from the extremophilic Streptomyces sp. S9 (XynAS9) possesses broad adaptability to temperature and pH and thus is an attractive candidate in industrial applications. In particular, the major products of XynAS9 are xylose and xylobiose, which enable the subsequent bioconversion to be carried out with higher efficiency. Therefore, the three-dimensional structure of XynAS9 and its catalytic machinery are of great interest. Here, recombinant XynAS9 protein was expressed in Pichia pastoris, purified and crystallized. Crystals belonging to the hexagonal space group P6(5)22, with unit-cell parameters a = b = 80.9, c = 289.3 angstrom, were obtained by the sittingdrop vapour-diffusion method and diffracted to 2.08 angstrom resolution. Initial phase determination using molecular replacement indicated that the crystal contains one molecule in an asymmetric unit. Further model building and structural refinement are in progress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | MICROBIAL XYLANASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329940900021 |
| 源URL | [http://124.16.173.210/handle/834782/1397]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Chinese Acad Agr Sci, Minist Agr, Key Lab Feed Biotechnol, Feed Res Inst, Beijing 100081, Peoples R China 4.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan |
| 推荐引用方式 GB/T 7714 | Lv, Pin,Zhang, Lilan,Luo, Huiying,et al. Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp S9[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2014,70:105-107. |
| APA | Lv, Pin.,Zhang, Lilan.,Luo, Huiying.,Chen, Chun-Chi.,Huang, Chun-Hsiang.,...&Guo, Rey-Ting.(2014).Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp S9.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,70,105-107. |
| MLA | Lv, Pin,et al."Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp S9".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 70(2014):105-107. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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