Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima
文献类型：期刊论文
| 作者 | He, Miao1; Zheng, Yingying2; Huang, Chun-Hsiang2; Qian, Guojun3; Xiao, Xiansha2; Ko, Tzu-Ping4; Shao, Weilan3; Guo, Rey-Ting2 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 70页码:1563-1565 |
| 英文摘要 | S-Adenosylhomocysteine hydrolase (SAHH) catalyzes the reversible conversion of S-adenosylhomocysteine into adenosine and homocysteine. The SAHH from Thermotoga maritima (TmSAHH) was expressed in Escherichia coli and the recombinant protein was purified and crystallized. TmSAHH crystals belonging to space group C2, with unit-cell parameters a = 106.3, b = 112.0, c = 164.9 angstrom, beta = 103.5 degrees, were obtained by the sitting-drop vapour-diffusion method and diffracted to 2.85 angstrom resolution. Initial phase determination by molecular replacement clearly indicated that the crystal contains one homotetramer per asymmetric unit. Further refinement of the crystal structure is in progress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | L-HOMOCYSTEINE HYDROLASE ; SITE-DIRECTED MUTAGENESIS ; CRYSTAL-STRUCTURE ; PLASMODIUM-FALCIPARUM ; CATALYTIC MECHANISM ; INHIBITORS ; ERITADENINE ; ANALOGS ; CLONING ; LIVER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344790900023 |
| 源URL | [http://124.16.173.210/handle/834782/1412]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China 4.Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan |
| 推荐引用方式 GB/T 7714 | He, Miao,Zheng, Yingying,Huang, Chun-Hsiang,et al. Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:1563-1565. |
| APA | He, Miao.,Zheng, Yingying.,Huang, Chun-Hsiang.,Qian, Guojun.,Xiao, Xiansha.,...&Guo, Rey-Ting.(2014).Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,1563-1565. |
| MLA | He, Miao,et al."Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):1563-1565. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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