Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor
文献类型：期刊论文
| 作者 | Zheng, Yingying1; Ko, Tzu-Ping2; Yang, Yunyun1,3; Shao, Weilan4; Guo, Rey-Ting1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2015-01-16 |
| 卷号 | 456期号:3页码:733-736 |
| 关键词 | Transcription regulation Ethanol fermentation Thennoanaerobacter ethanolicus Allosteric effector |
| 英文摘要 | The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/alpha-NAD(+) complex shows a different set of ligand interactions mainly due to the unique configuration of the nicotinamide moiety. The positively charged ring is covered by the Tyr102 side chain and interacts with a sulfate ion adjacent to the N-terminus of helix alpha 8. Consequently, the RSP dimer may be locked in a closed conformation that does not bind to DNA. However, alpha-NAD(+) does not show a higher affinity to RSP than B-NAD+. It has to be improved for possible use as an effector in modulating the repressor. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | REDOX ; CRYSTALLOGRAPHY ; FEATURES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348973300005 |
| 源URL | [http://124.16.173.210/handle/834782/1429]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 4.Jiangsu Univ, Biofuels Inst, Sch Environm, Zhenjiang 212013, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Yingying,Ko, Tzu-Ping,Yang, Yunyun,et al. Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,456(3):733-736. |
| APA | Zheng, Yingying,Ko, Tzu-Ping,Yang, Yunyun,Shao, Weilan,&Guo, Rey-Ting.(2015).Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,456(3),733-736. |
| MLA | Zheng, Yingying,et al."Binding mode of the oxidized alpha-anomer of NAD(+) to RSP, a Rex-family repressor".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456.3(2015):733-736. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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