Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum
文献类型：期刊论文
| 作者 | Yuan, Shuo-Fu2; Wu, Tzu-Hui3; Lee, Hsiao-Lin1; Hsieh, Han-Yu2; Lin, Wen-Ling1; Yang, Barbara2; Chang, Chih-Kang1; Li, Qian4; Gao, Jian4; Huang, Chun-Hsiang4 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2015-02-27 |
| 卷号 | 290期号:9页码:5739-5748 |
| 英文摘要 | We expressed an active form of CtCel5E (a bifunctional cellulase/xylanase from Clostridium thermocellum), performed biochemical characterization, and determined its apo- and ligand-bound crystal structures. From the structures, Asn-93, His-168, His-169, Asn-208, Trp-347, and Asn-349 were shown to provide hydrogen-bonding/hydrophobic interactions with both ligands. Compared with the structures of TmCel5A, a bifunctional cellulase/mannanase homolog from Thermotoga maritima, a flexible loop region in CtCel5E is the key for discriminating substrates. Moreover, site-directed mutagenesis data confirmed that His-168 is essential for xylanase activity, and His-169 is more important for xylanase activity, whereas Asn-93, Asn-208, Tyr-270, Trp-347, and Asn-349 are critical for both activities. In contrast, F267A improves enzyme activities. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HYDROLASE FAMILY 5 ; SUBSTRATE-SPECIFICITY ; THERMOTOGA-MARITIMA ; CRYSTAL-STRUCTURE ; GLYCOSYL HYDROLASES ; ENDOGLUCANASE ; COMPLEX ; POLYSACCHARIDES ; CLASSIFICATION ; CELLOTETRAOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350044200041 |
| 源URL | [http://124.16.173.210/handle/834782/1435]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 2.Natl Taiwan Univ, Inst Biochem Sci, Taipei 10617, Taiwan 3.Natl Taiwan Univ, Inst Biotechnol, Taipei 10617, Taiwan 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Shuo-Fu,Wu, Tzu-Hui,Lee, Hsiao-Lin,et al. Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(9):5739-5748. |
| APA | Yuan, Shuo-Fu.,Wu, Tzu-Hui.,Lee, Hsiao-Lin.,Hsieh, Han-Yu.,Lin, Wen-Ling.,...&Liang, Po-Huang.(2015).Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum.JOURNAL OF BIOLOGICAL CHEMISTRY,290(9),5739-5748. |
| MLA | Yuan, Shuo-Fu,et al."Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum".JOURNAL OF BIOLOGICAL CHEMISTRY 290.9(2015):5739-5748. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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