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  4. 期刊论文
Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum

文献类型：期刊论文

作者Chen, Chun-Chi1; Huang, Jian-Wen2; Zhao, Puya1; Ko, Tzu-Ping3; Huang, Chun-Hsiang1; Chan, Hsiu-Chien1; Huang, Zhiyong1; Liu, Wenting1; Cheng, Ya-Shan4,5; Liu, Je-Ruei2
刊名ENZYME AND MICROBIAL TECHNOLOGY
出版日期2015-04-01
卷号71期号:71页码:1-7
关键词beta-1,3-1,4-Glucanase Crystal structure Thermostability Industrial enzyme Pichia pastoris
英文摘要A thermophilic glycoside hydrolase family 16 (GH16)beta-1,3-1,4-glucanase from Clostridium thermocellum (CtLic16A) holds great potentials in industrial applications due to its high specific activity and outstanding thermostability. In order to understand its molecular machinery, the crystal structure of CtLic16A was determined to 1.95 angstrom resolution. The enzyme folds into a classic GH16 beta-jellyroll architecture which consists of two beta-sheets atop each other, with the substrate-binding cleft lying on the concave side of the inner beta-sheet. Two Bis-Tris propane molecules were found in the positive and negative substrate binding sites. Structural analysis suggests that the major differences between the CtLic16A and other GH16 beta-1,3-1,4-glucanase structures occur at the protein exterior. Furthermore, the high catalytic efficacy and thermal profile of the CtLic16A are preserved in the enzyme produced in Pichia pastoris, encouraging its further commercial applications. (C) 2015 Elsevier Inc. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]CRYSTAL-STRUCTURE ; BACILLUS 1,3-1,4-BETA-GLUCANASE ; FIBROBACTER-SUCCINOGENES ; 1,3-1,4-BETA-D-GLUCANASE ; 4-GLUCANOHYDROLASE ; RESOLUTION ; MACERANS ; ENZYMES ; PROTEIN
收录类别SCI
语种英语
WOS记录号WOS:000351794200001
源URL[http://124.16.173.210/handle/834782/1446]  
专题天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文
作者单位1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China
2.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan
3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan
4.Genozyme Biotechnol Inc, Taipei 106, Taiwan
5.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Chun-Chi,Huang, Jian-Wen,Zhao, Puya,et al. Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum[J]. ENZYME AND MICROBIAL TECHNOLOGY,2015,71(71):1-7.
APA Chen, Chun-Chi.,Huang, Jian-Wen.,Zhao, Puya.,Ko, Tzu-Ping.,Huang, Chun-Hsiang.,...&Guo, Rey-Ting.(2015).Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum.ENZYME AND MICROBIAL TECHNOLOGY,71(71),1-7.
MLA Chen, Chun-Chi,et al."Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum".ENZYME AND MICROBIAL TECHNOLOGY 71.71(2015):1-7.

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来源：天津工业生物技术研究所

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