Characterization of the sugar alcohol-producing yeast Pichia anomala
文献类型：期刊论文
| 作者 | Zhang, Guoqiang1; Lin, Yuping1; He, Peng2; Li, Lin1,3; Wang, Qinhong1; Ma, Yanhe1 |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 41期号:1页码:41-48 |
| 关键词 | Pichia anomala Sugar alcohol Bioconversion d-Arabitol dehydrogenase |
| 英文摘要 | Sugar alcohols have been widely applied in the field of food and medicine for their unique properties. Compared to chemical production, microbial production of sugar alcohol has become attractive for its environmental and sustainable pattern. In this study, a potential yeast isolated from soil of Beijing suburbs was identified as Pichia anomala TIB-x229, and its key enzyme of d-arabitol dehydrogenase for microbial production of sugar alcohols was functionally characterized. This yeast could simultaneously produce d-arabitol, xylitol, and/or ribitol from a different ratio of sugar substrates at a high efficiency by bioconversion, and no glucose repression happened when mixed sugars of xylose and glucose were used as the substrates during the bioconversion. This yeast could also efficiently convert complicated feedstock such as xylose mother liquor to d-arabitol, xylitol, and ribitol with 55 % yields. To elucidate the conversion relationship of the sugar alcohols, especially d-arabitol and xylitol, the key d-arabitol dehydrogenase gene from P. anomala was cloned, expressed and purified for further in vitro characterization. The results showed that this d-arabitol dehydrogenase could catalyze arabitol to xylulose further, which is significant for xylitol production from glucose. Our study laid the foundation for improving the production of sugar alcohols by metabolic and fermentation engineering strategies. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CANDIDA-TROPICALIS ; XYLITOL PRODUCTION ; D-ARABITOL ; XYLOSE REDUCTASE ; WATER-STRESS ; DEHYDROGENASE ; GLUCOSE ; BIOCONVERSION ; FERMENTATION ; ACCUMULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329324700005 |
| 源URL | [http://124.16.173.210/handle/834782/1378]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Ctr Microbial Biotechnol, Beijing 100080, Peoples R China 3.Jangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Guoqiang,Lin, Yuping,He, Peng,et al. Characterization of the sugar alcohol-producing yeast Pichia anomala[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2014,41(1):41-48. |
| APA | Zhang, Guoqiang,Lin, Yuping,He, Peng,Li, Lin,Wang, Qinhong,&Ma, Yanhe.(2014).Characterization of the sugar alcohol-producing yeast Pichia anomala.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,41(1),41-48. |
| MLA | Zhang, Guoqiang,et al."Characterization of the sugar alcohol-producing yeast Pichia anomala".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 41.1(2014):41-48. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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