Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Shui, Wenqing1; Xiong, Yun1; Xiao, Weidi2,3; Qi, Xianni1; Zhang, Yong2,3; Lin, Yuping1; Guo, Yufeng1; Zhang, Zhidan1; Wang, Qinhong1; Ma, Yanhe1 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 14期号:7页码:1885-1897 |
| 英文摘要 | Saccharomyces cerevisiae has been intensively studied in responses to different environmental stresses such as heat shock through global omic analysis. However, the S. cerevisiae industrial strains with superior thermotolerance have not been explored in any proteomic studies for elucidating the tolerance mechanism. Recently a new diploid strain was obtained through evolutionary engineering of a parental industrial strain, and it exhibited even higher resistance to prolonged thermal stress. Herein, we performed iTRAQ-based quantitative proteomic analysis on both the parental and evolved industrial strains to further understand the mechanism of thermotolerant adaptation. Out of similar to 2600 quantifiable proteins from biological quadruplicates, 193 and 204 proteins were differentially regulated in the parental and evolved strains respectively during heat-stressed growth. The proteomic response of the industrial strains cultivated under prolonged thermal stress turned out to be substantially different from that of the laboratory strain exposed to sudden heat shock. Further analysis of transcription factors underlying the proteomic perturbation also indicated the distinct regulatory mechanism of thermotolerance. Finally, a cochaperone Mdj1 and a metabolic enzyme Adh1 were selected to investigate their roles in mediating heat-stressed growth and ethanol production of yeasts. Our proteomic characterization of the industrial strain led to comprehensive understanding of the molecular basis of thermotolerance, which would facilitate future improvement in the industrially important trait of S. cerevisiae by rational engineering. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | GENE-EXPRESSION ; OXIDATIVE STRESS ; YEAST-CELLS ; ENVIRONMENTAL-CHANGES ; MOLECULAR CHAPERONES ; TRANSCRIPTION FACTOR ; ETHANOL-PRODUCTION ; BUDDING YEAST ; PROTEIN ; TEMPERATURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357434400012 |
| 源URL | [http://124.16.173.210/handle/834782/1501]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shui, Wenqing,Xiong, Yun,Xiao, Weidi,et al. Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(7):1885-1897. |
| APA | Shui, Wenqing.,Xiong, Yun.,Xiao, Weidi.,Qi, Xianni.,Zhang, Yong.,...&Ma, Yanhe.(2015).Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae.MOLECULAR & CELLULAR PROTEOMICS,14(7),1885-1897. |
| MLA | Shui, Wenqing,et al."Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae".MOLECULAR & CELLULAR PROTEOMICS 14.7(2015):1885-1897. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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