Engineering catechol 1,2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli
文献类型：期刊论文
| 作者 | Han, Li1; Liu, Pi2,3; Sun, Jixue2,3; Wu, Yuanqing1; Zhang, Yuanyuan1; Chen, Wujiu1; Lin, Jianping1,2,3; Wang, Qinhong1; Ma, Yanhe1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-08-26 |
| 卷号 | 5 |
| 英文摘要 | Regulating and ameliorating enzyme expression and activity greatly affects the performance of a given synthetic pathway. In this study, a new synthetic pathway for cis, cis-muconic acid (ccMA) production was reconstructed without exogenous induction by regulating the constitutive expression of the important enzyme catechol 1,2-dioxygenase (CatA). Next, new CatAs with significantly improved activities were developed to enhance ccMA production using structure-assisted protein design. Nine mutations were designed, simulated and constructed based on the analysis of the CatA crystal structure. These results showed that mutations at Gly72, Leu73 and/or Pro76 in CatA could improve enzyme activity, and the activity of the most effective mutant was 10-fold greater than that of the wild-type CatA from Acinetobacter sp. ADP1. The most productive synthetic pathway with a mutated CatA increased the titer of ccMA by more than 25%. Molecular dynamic simulation results showed that enlarging the entrance of the substrate-binding pocket in the mutants contributed to their increased enzyme activities and thus improved the performance of the synthetic pathway. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CIS,CIS-MUCONIC ACID ; ADIPIC ACID ; BIOTECHNOLOGICAL PRODUCTION ; SACCHAROMYCES-CEREVISIAE ; BIOSYNTHESIS ; PRECURSORS ; BENZOATE ; FUTURE ; TOOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360062500001 |
| 源URL | [http://124.16.173.210/handle/834782/1516]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Li,Liu, Pi,Sun, Jixue,et al. Engineering catechol 1,2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Han, Li.,Liu, Pi.,Sun, Jixue.,Wu, Yuanqing.,Zhang, Yuanyuan.,...&Ma, Yanhe.(2015).Engineering catechol 1,2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli.SCIENTIFIC REPORTS,5. |
| MLA | Han, Li,et al."Engineering catechol 1,2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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