TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes
文献类型：期刊论文
| 作者 | Zhang, Guoqiang1,2; Lin, Yuping1; Qi, Xianni1; Li, Lin1; Wang, Qinhong1; Ma, Yanhe1 |
| 刊名 | ACS SYNTHETIC BIOLOGY
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 4期号:10页码:1101-1111 |
| 关键词 | transcription activator-like effector nucleases multiplex editing genome evolution cellular phenotypes |
| 英文摘要 | Genome editing is an important tool for building novel genotypes with a desired phenotype. However, the fundamental challenge is to rapidly generate desired alterations on a genome-wide scale. Here, we report TALENs (transcription activator-like effector nucleases)-assisted multiplex editing (TAME), based on the interaction of designed TALENs with the DNA sequences between the critical TATA and GC boxes, for generating multiple targeted genomic modifications. Through iterative cycles of TAME to induce abundant semirational indels coupled with efficient screening using a reporter, the targeted fluorescent trait can be continuously and rapidly improved by accumulating multiplex beneficial genetic modifications in the evolving yeast genome. To further evaluate its efficiency, we also demonstrate the application of TAME for significantly improving ethanol tolerance of yeast in a short amount of time. Therefore, TAME is a broadly generalizable platform for accelerated genome evolution to rapidly improve yeast phenotypes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; EFFECTOR NUCLEASES ; GENE-EXPRESSION ; TRANSCRIPTION FACTORS ; DNA-BINDING ; IDENTIFICATION ; COMPLEX ; CELLS ; PROMOTER ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363226700007 |
| 源URL | [http://124.16.173.210/handle/834782/1560]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Guoqiang,Lin, Yuping,Qi, Xianni,et al. TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes[J]. ACS SYNTHETIC BIOLOGY,2015,4(10):1101-1111. |
| APA | Zhang, Guoqiang,Lin, Yuping,Qi, Xianni,Li, Lin,Wang, Qinhong,&Ma, Yanhe.(2015).TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes.ACS SYNTHETIC BIOLOGY,4(10),1101-1111. |
| MLA | Zhang, Guoqiang,et al."TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes".ACS SYNTHETIC BIOLOGY 4.10(2015):1101-1111. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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