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Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions

文献类型：期刊论文

作者Jiang, Xu1,2; Xue, Yanfen3; Wang, Aiyan1; Wang, Limin1; Zhang, Guimin2; Zeng, Qingtao2; Yu, Bo1; Ma, Yanhe3,4
刊名BIORESOURCE TECHNOLOGY
出版日期2013-09-01
卷号143页码:665-668
关键词L-Lactate Alkaliphile Exiguobacterium sp. Open fermentation
英文摘要In this study, a newly isolated alkaliphile Exiguobacterium sp. strain 8-11-1 was used to produce optically pure L-lactate. With an initial glucose concentration of 80 g/L, a high overall L-lactic acid productivity of 8.15 g/L/h was achieved using NaOH as a neutralizing agent. The fed-batch fermentations were carried out under both sterile and nonsterile conditions. Under the nonsterile condition, 125 g/L L-lactic acid was obtained with a high percent yield and average productivity of 98.33% and 3.79 g/L/h, respectively. These values were consistent with the results from sterile condition. No D-isomers of lactic acid were detected, resulting in an optical purity of 100% in both conditions. The high levels of optically pure L-lactic acid produced by Exiguobacterium sp. 8-11-1, combined with the ease of handling and low costs associated with the open fermentation strategy, provide a novel and potentially important approach for L-lactic acid production in the future. (C) 2013 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]HIGH-OPTICAL-PURITY ; L-LACTIC ACID
收录类别SCI
语种英语
WOS记录号WOS:000323239800089
源URL[http://124.16.173.210/handle/834782/1254]  
专题天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文
作者单位1.Chinese Acad Sci, CAS Key Lab Microbial Physiol & Metab Engn, Inst Microbiol, Beijing 100101, Peoples R China
2.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China
3.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Xu,Xue, Yanfen,Wang, Aiyan,et al. Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions[J]. BIORESOURCE TECHNOLOGY,2013,143:665-668.
APA Jiang, Xu.,Xue, Yanfen.,Wang, Aiyan.,Wang, Limin.,Zhang, Guimin.,...&Ma, Yanhe.(2013).Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions.BIORESOURCE TECHNOLOGY,143,665-668.
MLA Jiang, Xu,et al."Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp strain under nonsterile open fermentation conditions".BIORESOURCE TECHNOLOGY 143(2013):665-668.

入库方式： OAI收割

来源：天津工业生物技术研究所

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