Bioassay-Guided Isolation of DPP-4 Inhibitory Fractions from Extracts of Submerged Cultured of Inonotus obliquus
文献类型：期刊论文
| 作者 | Geng, Yan1; Lu, Zhen-Ming1; Huang, Wei1; Xu, Hong-Yu1; Shi, Jin-Song1; Xu, Zheng-Hong1,2 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2013 |
| 卷号 | 18期号:1页码:1150-1161 |
| 关键词 | Inonotus obliquus DPP-4 inhibitor bioassay-guided isolation UPLC-Q-TOF-MS molecular docking |
| 英文摘要 | Inonotus obliquus is a medicinal mushroom used in Russian and Eastern European folk medicine for the treatment of gastrointestinal cancer, cardiovascular disease and diabetes. Previous studies in our laboratory have demonstrated that the mycelium powders of I. obliquus possess significant antihyperglycemic effects in a mouse model of diabetic disease induced by alloxan. However, the active ingredients of mycelium powders responsible for the diabetes activity have not been identified. This study aims to identify the active ingredients of I. obliquus mycelium powders by a bioassay-guided fractionation approach and explore the mechanism of action of these active ingredients by using a well-established DPP-4 (an important enzyme as a new therapeutic target for diabetes) inhibitory assay model. The results showed the chloroform extract of mycelium was potential inhibitory against DPP-4. Bioactivity guided fractionation led to the identification of 19 compounds using UPLC-Q-TOF-MS. Molecular docking between the compounds and DPP-4 revealed that compounds 5, 8, 9, 14, 15 may be the active components responsible for the DPP-4 inhibitory activity. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Organic |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | CHROMATOGRAPHY-MASS-SPECTROMETRY ; TYPE-2 DIABETES-MELLITUS ; PILAT APHYLLOPHOROMYCETIDEAE ; ANTIOXIDANT ACTIVITIES ; CHEMICAL-CONSTITUENTS ; MEDICINAL MUSHROOM ; DRY-MATTER ; CHAGA ; MICE ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314032900073 |
| 源URL | [http://124.16.173.210/handle/834782/1291]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Jiangsu, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Yan,Lu, Zhen-Ming,Huang, Wei,et al. Bioassay-Guided Isolation of DPP-4 Inhibitory Fractions from Extracts of Submerged Cultured of Inonotus obliquus[J]. MOLECULES,2013,18(1):1150-1161. |
| APA | Geng, Yan,Lu, Zhen-Ming,Huang, Wei,Xu, Hong-Yu,Shi, Jin-Song,&Xu, Zheng-Hong.(2013).Bioassay-Guided Isolation of DPP-4 Inhibitory Fractions from Extracts of Submerged Cultured of Inonotus obliquus.MOLECULES,18(1),1150-1161. |
| MLA | Geng, Yan,et al."Bioassay-Guided Isolation of DPP-4 Inhibitory Fractions from Extracts of Submerged Cultured of Inonotus obliquus".MOLECULES 18.1(2013):1150-1161. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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