Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans
文献类型：期刊论文
| 作者 | Yue, Yang1,2; Liu, Shengquan3; Li, Hongbin2; Song, Binghong2; Xie, Ting2; Sun, Yan1; Chao, Yapeng2; Qian, Shijun2 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2013-10-01 |
| 卷号 | 69页码:1186-1189 |
| 英文摘要 | Improving the specificity of alpha-cyclodextrin glucanotransferase is a significant issue in the field of alpha-cyclodextrin production. In this study, a constructed Y167H mutant alpha-cyclodextrin glucanotransferase with enhanced alpha-cyclodextrin specificity was successfully expressed and purified. Single crystals were grown using PEG 4000 as a precipitating agent by the hanging-drop vapour-diffusion method at 293 K. The crystals exhibited two kinds of morphology in different crystallization conditions. The crystals diffracted to at least 2.2 A resolution (space group P2(1)2(1)2(1)), with unit-cell parameters a = 65.69, b = 78.70, c = 137.00 angstrom. Assuming the asymmetric cell to be occupied by a monomer of 75 kDa, the unit cell contains 43.77% solvent with a crystal volume per protein mass, V-M, of 2.19 angstrom(3) Da(-1) |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | ANGSTROM RESOLUTION ; PRODUCT SPECIFICITY ; GLYCOSYLTRANSFERASE ; CIRCULANS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325402900028 |
| 源URL | [http://124.16.173.210/handle/834782/1300]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Beihang Univ BUAA, Sch Biol Sci & Med Engn, Minist Educ, Key Lab Biomech & Mechanobiol, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, State Key Labs Transducer Technol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Yang,Liu, Shengquan,Li, Hongbin,et al. Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:1186-1189. |
| APA | Yue, Yang.,Liu, Shengquan.,Li, Hongbin.,Song, Binghong.,Xie, Ting.,...&Qian, Shijun.(2013).Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,1186-1189. |
| MLA | Yue, Yang,et al."Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):1186-1189. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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