Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca
文献类型：期刊论文
| 作者 | Zhang, Gang1,2; Yang, Guang3; Wang, Xu1,2; Guo, Qingjuan1,2; Li, Ying1,2; Li, Jilun1,2 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 168期号:1页码:116-128 |
| 关键词 | 1,3-Propanediol 2,3-Butanediol alpha-Acetolactate decarboxylase Reducing equivalent Klebsiella oxytoca |
| 英文摘要 | Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water solubility with 1,3-PD, not only contests the carbon flow and NADH with 1,3-PD but also serves as an obstacle for obtaining high purity 1,3-PD in downstream processes. In this study, a 2,3-BD pathway-deficient mutant of Klebsiella oxytoca ZG36 was constructed by knocking out the budA gene of the wild-type strain M5al. The results of fed-batch fermentation by ZG36 indicated that the glycerol flux and the distribution of metabolites were altered in the K. oxytoca when the 2,3-BD pathway was blocked. No 2,3-BD was produced, and the activity of alpha-acetolactate decarboxylase (alpha-ALDC) can not be detected in the fermentation processes. The indexes of the 1,3-PD titer, the conversion from glycerol to 1,3-PD, and the productivity per cell dry weight (CDW) increased by 42%, 62%, and 46%, respectively, compared with the M5al, and the yield of the byproducts also increased obviously. The assay of the enzyme activities in the oxidative branch and the reductive branch of the glycerol metabolism, as well as the intracellular redox state, exposited the results logically. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | ANAEROBIC CONTINUOUS-CULTURE ; PNEUMONIAE ; FERMENTATION ; DEHYDROGENASE ; INACTIVATION ; ELIMINATION ; ENZYMES ; FLUXES ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309223700012 |
| 源URL | [http://124.16.173.210/handle/834782/1310]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Beijing 100193, Peoples R China 2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Gang,Yang, Guang,Wang, Xu,et al. Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,168(1):116-128. |
| APA | Zhang, Gang,Yang, Guang,Wang, Xu,Guo, Qingjuan,Li, Ying,&Li, Jilun.(2012).Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,168(1),116-128. |
| MLA | Zhang, Gang,et al."Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 168.1(2012):116-128. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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