Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5
文献类型：期刊论文
| 作者 | Bai, Wenqin1,2; Xue, Yanfen1; Zhou, Cheng1; Ma, Yanhe1 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 34期号:11页码:2093-2099 |
| 关键词 | Bacillus sp SN5 Cold-active Halo-tolerant Xylanase |
| 英文摘要 | A xylanase gene (xyn10A) was cloned from Bacillus sp. SN5 and expressed in Escherichia coli. It encoded a 348-residue polypeptide of similar to 45 kDa. The deduced amino acid sequence had 68 % identity with the endo-1,4-beta-xylanase from Paenibacillus lactis 154 that belonged to family 10 of the glycoside hydrolases. Purified recombinant Xyn10A had maximum activity at 40 degrees C and pH 7.0, with the specific activity of 105 U/mg and a Km of 0.6 mg/ml for beechwood xylan. Xyn10A retained more than 80 % activity between 25 and 45 degrees C and 29 % activity at 5 degrees C. It exhibited the highest activity (134 %) in 0.5 M NaCl and still retained 90 % activity in 2.5 M NaCl. It retained about 87 % activity after incubation in 2 M NaCl for 24 h. The cold-active and halo-tolerant properties of Xyn10A make it promising for application in the food industry, especially in the processing of saline food and sea food. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | THERMOSTABLE XYLANASE ; STRAIN ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310587100018 |
| 源URL | [http://124.16.173.210/handle/834782/1322]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Wenqin,Xue, Yanfen,Zhou, Cheng,et al. Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5[J]. BIOTECHNOLOGY LETTERS,2012,34(11):2093-2099. |
| APA | Bai, Wenqin,Xue, Yanfen,Zhou, Cheng,&Ma, Yanhe.(2012).Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5.BIOTECHNOLOGY LETTERS,34(11),2093-2099. |
| MLA | Bai, Wenqin,et al."Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp SN5".BIOTECHNOLOGY LETTERS 34.11(2012):2093-2099. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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