Engineering cellular robustness of microbes by introducing the GroESL chaperonins from extremophilic bacteria
文献类型：期刊论文
| 作者 | Luan, Guodong1,2; Dong, Hongjun1; Zhang, Tianrui3; Lin, Zhao1,2; Zhang, Yanping1; Li, Yin1; Cai, Zhen1 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2014-05-20 |
| 卷号 | 178页码:38-40 |
| 关键词 | GroESL Cellular robustness Clostridium acetobutylicum |
| 英文摘要 | The cellular robustness is a big concern for efficient microbial production of biofuels and biochemicals. In this study, the groESL genes from extremophilic bacteria were found to serve as transplantable stress-response elements to improve diverse types of stress-tolerances of other microbes. By overexpressing the groESL from the solvent-tolerant Pseudomonas putida in Escherichia coli, its thermo-tolerance and ethanol-tolerance were significantly increased. Meanwhile, the groESL from the thermophilic Thermoanaerobacter tengcongensis endowed Clostridium acetobutylicum with improved corn cob hydrolysates (CCH)-tolerance as well as elevated butanol productivity. The chaperonins GroESL have been widely considered as cellular stress-response proteins and overexpression of native groESL has been proven to improve cellular tolerances facing various stresses. Here we found that the groESL genes from extremophilic bacteria were superior to the native ones, possibly because they have adapted to the environmental stresses during long-term natural evolution. Moreover, our results also revealed that different extreme groESL genes performed quite different in different microbes. Thus the relation and compatibility between the extremophiles and the host must be considered for selection of the proper groESL for engineering microbial robustness. (c) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | LACTOBACILLUS-PARACASEI NFBC-338 ; ESCHERICHIA-COLI ; CLOSTRIDIUM-ACETOBUTYLICUM ; STRESS TOLERANCE ; SOLVENT PRODUCTION ; GLOBAL REGULATOR ; OVEREXPRESSION ; STRAIN ; FERMENTATION ; INHIBITORS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335557200005 |
| 源URL | [http://124.16.173.210/handle/834782/1381]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, State Engn Lab Ind Enzymes, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Guodong,Dong, Hongjun,Zhang, Tianrui,et al. Engineering cellular robustness of microbes by introducing the GroESL chaperonins from extremophilic bacteria[J]. JOURNAL OF BIOTECHNOLOGY,2014,178:38-40. |
| APA | Luan, Guodong.,Dong, Hongjun.,Zhang, Tianrui.,Lin, Zhao.,Zhang, Yanping.,...&Cai, Zhen.(2014).Engineering cellular robustness of microbes by introducing the GroESL chaperonins from extremophilic bacteria.JOURNAL OF BIOTECHNOLOGY,178,38-40. |
| MLA | Luan, Guodong,et al."Engineering cellular robustness of microbes by introducing the GroESL chaperonins from extremophilic bacteria".JOURNAL OF BIOTECHNOLOGY 178(2014):38-40. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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