Constitutive expression of alkaline beta-mannanase in recombinant Pichia pastoris
文献类型：期刊论文
| 作者 | Zhu, Taicheng1; Sun, Hongbing2; Li, Pengfei1; Xue, Yanfen3; Li, Yin1; Ma, Yanhe2,3 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 49期号:12页码:2025-2029 |
| 关键词 | Pichia pastoris Alkaline beta-mannanase Constitutive expression Chaperone |
| 英文摘要 | Alkaline beta-mannanase has important applications for specific industrial processes like pulp bleaching and the detergent industry. The low yield of alkaline beta-mannanase produced from native microbes such as alkaliphilic Bacillus limits its applications. Pichia pastoris is the most efficient heterologous system to produce alkaline mannanase. However, the previous use of the AOX system required large amount of methanol and sophisticated operation strategy, which are undesirable in large scale production. In this study, we established a safe and simple constitutive expression process for mannanase production in P. pastoris. The mannanase gene was successfully expressed under the control of GAP promoter. Sequential optimization of the constructed strains was also performed including the copy number optimization and co-expression of chaperone genes. A two-stage feeding strategy was then applied for the finally optimized strain. After 96 h fermentation, a production level of 2980 U/mL was finally reached, illustrating the potential of the GAP constitutive expression system for industrial scale preparation of alkaline beta-mannanase. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | BACILLUS SP N-16-5 ; PROTEIN-PRODUCTION ; GENE ; PROMOTER ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000347761100001 |
| 源URL | [http://124.16.173.210/handle/834782/1383]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Taicheng,Sun, Hongbing,Li, Pengfei,et al. Constitutive expression of alkaline beta-mannanase in recombinant Pichia pastoris[J]. PROCESS BIOCHEMISTRY,2014,49(12):2025-2029. |
| APA | Zhu, Taicheng,Sun, Hongbing,Li, Pengfei,Xue, Yanfen,Li, Yin,&Ma, Yanhe.(2014).Constitutive expression of alkaline beta-mannanase in recombinant Pichia pastoris.PROCESS BIOCHEMISTRY,49(12),2025-2029. |
| MLA | Zhu, Taicheng,et al."Constitutive expression of alkaline beta-mannanase in recombinant Pichia pastoris".PROCESS BIOCHEMISTRY 49.12(2014):2025-2029. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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