Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle
文献类型：期刊论文
| 作者 | Li, Z.1,2; Huang, H.1; Zhao, H.1; Meng, K.1; Zhao, J.3; Shi, P.1; Yang, P.1; Luo, H.1; Wang, Y.1; Yao, B.1 |
| 刊名 | LETTERS IN APPLIED MICROBIOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 59期号:6页码:615-620 |
| 关键词 | clone library cysteine phytase rumen Small Tail Han sheep transcriptional profile |
| 英文摘要 | Cysteine phytase is the main phytate-degrading enzyme of ruminant animals. To explore the genetic diversity and dynamic expression profile of cysteine phytase in sheep rumen during a feeding cycle, four transcript (0, 4, 9 and 16h after feeding) and one DNA (9h after feeding) clone libraries were constructed, respectively. A total of 46 distinct gene fragments were identified, and most of these sequences had low identities (<60%) with known phytases. Great divergence was found in the constitution and abundance of genes at the genome and transcriptional levels, and the transcript data are more reliable to reflect the information of functional genes. Phylogenetic analysis indicated that the genes from uncultured bacteria instead of Firmicutes played the major phytate-degrading role. Further comparative analysis revealed the dynamic constitution of cysteine phytase genes in rumen at different time points. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | PROGRAM ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345218100008 |
| 源URL | [http://124.16.173.210/handle/834782/1388]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Tianjin, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Z.,Huang, H.,Zhao, H.,et al. Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle[J]. LETTERS IN APPLIED MICROBIOLOGY,2014,59(6):615-620. |
| APA | Li, Z..,Huang, H..,Zhao, H..,Meng, K..,Zhao, J..,...&Yao, B..(2014).Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle.LETTERS IN APPLIED MICROBIOLOGY,59(6),615-620. |
| MLA | Li, Z.,et al."Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle".LETTERS IN APPLIED MICROBIOLOGY 59.6(2014):615-620. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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