Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme
文献类型：期刊论文
| 作者 | Ge, Chenchen1,3; Luo, Quan2; Wang, Dou1; Zhao, Shiming1,4; Liang, Xiaoling1; Yu, Luxin1; Xing, Xuerong5; Zeng, Lingwen1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 86期号:13页码:6387-6392 |
| 英文摘要 | G-quadruplex-forming sequence can be formed through a copper(I) ion (Cu+)-catalyzed click chemistry between azide- and alkyne-modified short G-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. The source for Cu+ was generated from the reduction of Cu2+ with the reductant of sodium ascorbate. In the presence of hemin and K+, the self-assembly of hemin/G-quadruplex structure has the activity of horseradish peroxidase (HRP), which can catalyze its colorless substrate tetrazmethyl benzidine (TMB) into a colored product. Hence, the concentration of Cu2+ can be evaluated visually for qualitative analysis according to the color change of the solution, and the optical density (OD) value of the resulting solution at 450 nm was also recorded using a microplate reader for quantitative analysis. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | UNMODIFIED GOLD NANOPARTICLES ; VISUAL DETECTION ; CU2+ DETECTION ; ALZHEIMERS-DISEASE ; FLUORESCENT SENSOR ; HIGH-SENSITIVITY ; AQUEOUS-SOLUTION ; ENZYME-FREE ; METAL-IONS ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000338488800032 |
| 源URL | [http://124.16.173.210/handle/834782/1398]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China 2.Guangzhou Inst Dermatol, Guangzhou 510095, Guangdong, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China 4.Anhui Univ, Sch Life Sci, Hefei 230601, Peoples R China 5.Univ Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Chenchen,Luo, Quan,Wang, Dou,et al. Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme[J]. ANALYTICAL CHEMISTRY,2014,86(13):6387-6392. |
| APA | Ge, Chenchen.,Luo, Quan.,Wang, Dou.,Zhao, Shiming.,Liang, Xiaoling.,...&Zeng, Lingwen.(2014).Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme.ANALYTICAL CHEMISTRY,86(13),6387-6392. |
| MLA | Ge, Chenchen,et al."Colorimetric Detection of Copper(II) Ion Using Click Chemistry and Hemin/G-Quadruplex Horseradish Peroxidase-Mimicking DNAzyme".ANALYTICAL CHEMISTRY 86.13(2014):6387-6392. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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