Screening and Isolation for Anti-hepatofibrotic Components from Medicinal Mushrooms using TGF-beta 1-induced Live Fibrosis in Hepatic Stellate Cells
文献类型：期刊论文
| 作者 | Geng, Yan1; Wang, Jing1; Xie, Minfeng1; Lu, Zhen-Ming1; Xu, Hong-Yu1; Shi, Jin-Song1; Xu, Zheng-Hong1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
 |
| 出版日期 | 2014 |
| 卷号 | 16期号:6页码:529-539 |
| 关键词 | medicinal mushrooms liver fibrosis cell model picro-sirius red staining TGF-beta 1 anti-fibrotic activity Antrodia camphorata |
| 英文摘要 | Liver fibrosis is a wound-healing response to chronic liver injury that could lead to liver failure, but treatment remains ineffective. In this study, we investigated anti-hepatic fibrosis activity of n-hexane, chloroform, ethyl acetate, and methanol extracts of mycelia from six commercially available medicinal mushrooms in submerged culture, namely Antrodia camphorata, Cephalosporium sinensis, Cordyceps mortierella, Hericium erinaceus, Ganoderma lucidum, and Armillaria mellea. Their anti-fibrotic activities were evaluated via inhibition against accumulation of TGF-beta 1 induced collagen deposition in CFSC-8B cells. Hex, Chl, and MeOH extracts of A. camphorata and Hex extract of A. mellea significantly decreased collagen production. Bioactivity-guided fractionation led to the identification of seven compounds using UPLC-Q-TOF-MS from the Hex Fr.2 of A. camphorata. At the molecular level, Hex Fr.2 of A. camphorata suppressed alpha-SMA, Collagen I, Collagen III, and Fibronectin expression induced by TGF-beta 1 in CFSC-8B cells as indicated by qRT-PCR analysis. They also inhibited a-SMA and Collagen I protein expression according to western blot analyses. Mechanistically, Hex Fr.2 of A. camphorata negatively regulates TGF-beta 1/Smad2/3 signaling. Our studies demonstrate that A. camphorata has in vitro anti-hepatofibrotic activity and that there is great potential for the discovery of new drugs for the treatment of liver fibrosis by screening more medicinal mushrooms. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences ; Mycology ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences ; Mycology ; Pharmacology & Pharmacy |
| 关键词[WOS] | GROWTH-FACTOR-BETA ; TGF-BETA ; FIBROGENESIS ; REGENERATION ; MECHANISMS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344202200003 |
| 源URL | [http://124.16.173.210/handle/834782/1419]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Yan,Wang, Jing,Xie, Minfeng,et al. Screening and Isolation for Anti-hepatofibrotic Components from Medicinal Mushrooms using TGF-beta 1-induced Live Fibrosis in Hepatic Stellate Cells[J]. INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,2014,16(6):529-539. |
| APA | Geng, Yan.,Wang, Jing.,Xie, Minfeng.,Lu, Zhen-Ming.,Xu, Hong-Yu.,...&Xu, Zheng-Hong.(2014).Screening and Isolation for Anti-hepatofibrotic Components from Medicinal Mushrooms using TGF-beta 1-induced Live Fibrosis in Hepatic Stellate Cells.INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,16(6),529-539. |
| MLA | Geng, Yan,et al."Screening and Isolation for Anti-hepatofibrotic Components from Medicinal Mushrooms using TGF-beta 1-induced Live Fibrosis in Hepatic Stellate Cells".INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS 16.6(2014):529-539. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。