A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)
文献类型：期刊论文
| 作者 | Singh, Baneshwar1,2; Crippen, Tawni L.3; Zheng, Longyu1,3,4; Fields, Andrew T.1,5; Yu, Ziniu4; Ma, Qun6; Wood, Thomas K.7; Dowd, Scot E.8; Flores, Micah1,9; Tomberlin, Jeffery K.1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 99期号:2页码:869-883 |
| 关键词 | Microbial community Blow flies Maggot debridement therapy Salivary gland 454 sequencing |
| 英文摘要 | Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RIBOSOMAL-RNA SEQUENCES ; MAGGOT THERAPY ; FLIES DIPTERA ; STAPHYLOCOCCUS-AUREUS ; ANTIBIOTIC-RESISTANT ; FORENSIC ENTOMOLOGY ; MAXIMUM-LIKELIHOOD ; BIOFILM FORMATION ; DIGESTIVE-TRACT ; MUSCA-DOMESTICA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348770900027 |
| 源URL | [http://124.16.173.210/handle/834782/1424]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA 2.Virginia Commonwealth Univ, Dept Forens Sci, Richmond, VA 23284 USA 3.ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX USA 4.Huazhong Agr Univ, Natl Engn Res Ctr Microbe Pesticide, State Key Lab Agr Microbiol, Wuhan, Peoples R China 5.SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA 6.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 7.Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA 8.MR DNA Mol Res LP, Shallowater, TX USA 9.Walter Reed Army Inst Res, Silver Spring, MD USA |
| 推荐引用方式 GB/T 7714 | Singh, Baneshwar,Crippen, Tawni L.,Zheng, Longyu,et al. A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(2):869-883. |
| APA | Singh, Baneshwar.,Crippen, Tawni L..,Zheng, Longyu.,Fields, Andrew T..,Yu, Ziniu.,...&Tarone, Aaron M..(2015).A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae).APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(2),869-883. |
| MLA | Singh, Baneshwar,et al."A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.2(2015):869-883. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。