Recombinant Expression and Characterization of Thermoanaerobacter tengcongensis Thermostable alpha-Glucosidase with Regioselectivity for High-Yield Isomaltooligosaccharides Synthesis
文献类型：期刊论文
| 作者 | Zhou Cheng1,2; Xue, Yanfen1; Zhang, Yueling3; Zeng, Yan1; Ma, Yanhe1 |
| 刊名 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 19期号:12页码:1547-1556 |
| 关键词 | Thermostable alpha-glucosidase Thermoanaerobacter tengcongensis maltooligosaccharides hydrolysis isomaltooligosaccharides synthesis |
| 英文摘要 | A novel thermostable alpha-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in E. coli and characterized. The TtgluA gene contained 2,253 bp, which encodes 750 amino acids. The native TtGluA was a trimer with monomer molecular mass of 89 kDa shown by SDS-PAGE. The purified recombinant enzyme showed hydrolytic activity on maltooligosaccharides, p-nitrophenyl-alpha-D-glucopyranide, and dextrin with an exotype cleavage manner. TtGluA showed preference for short-chain maltooligosaccharides and the highest specific activity for maltose of 3.26 units/mg. Maximal activity was observed at 60 degrees C and pH 5.5. The half-life was 2 h at 60 degrees C. The enzyme showed good tolerance to urea and SDS but was inhibited by Tris. When maltose with the concentration over 50 mM was used as substrate, TtGluA was also capable of catalyzing transglycosylation to produce alpha-1,4-linked maltotriose and alpha-1,6-linked isomaltooligosaccharides. More importantly, TtGluA showed exclusive regiospecificity with high yield to produce alpha-1,6-linked isomaltooligosaccharides when the reaction time extended to more than 10 h. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | TRANSGLUCOSYLATION ACTIVITY ; CLOSTRIDIUM ISOLATE ; AMYLOLYTIC ENZYMES ; OLIGOSACCHARIDES ; PURIFICATION ; STARCH ; GLUCOAMYLASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273134800008 |
| 源URL | [http://124.16.173.210/handle/834782/1464]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Cheng,Xue, Yanfen,Zhang, Yueling,et al. Recombinant Expression and Characterization of Thermoanaerobacter tengcongensis Thermostable alpha-Glucosidase with Regioselectivity for High-Yield Isomaltooligosaccharides Synthesis[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2009,19(12):1547-1556. |
| APA | Zhou Cheng,Xue, Yanfen,Zhang, Yueling,Zeng, Yan,&Ma, Yanhe.(2009).Recombinant Expression and Characterization of Thermoanaerobacter tengcongensis Thermostable alpha-Glucosidase with Regioselectivity for High-Yield Isomaltooligosaccharides Synthesis.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,19(12),1547-1556. |
| MLA | Zhou Cheng,et al."Recombinant Expression and Characterization of Thermoanaerobacter tengcongensis Thermostable alpha-Glucosidase with Regioselectivity for High-Yield Isomaltooligosaccharides Synthesis".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 19.12(2009):1547-1556. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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