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Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline beta-mannanase in Pichia pastoris

文献类型：期刊论文

作者Zhu, Taicheng; You, Lijin; Gong, Fuyu; Xie, Minfeng; Xue, Yanfen1,2; Li, Yin1; Ma, Yanhe1,2
刊名ENZYME AND MICROBIAL TECHNOLOGY
出版日期2011-09-10
卷号49期号:4页码:407-412
关键词Multi-copy Pichia pastoris Alkaline mannanase High-level production Co-feeding
英文摘要A process for efficient production of an alkaline beta-mannanases from Bacillus sp. N16-5 was established by heterologous expression using Pichia pastoris. A high producing strain was generated by removing the native beta-mannanases signal peptide and increasing the copy number of the mature beta-mannanases gene. High cell density fermentation of this strain in 1-L bioreactor led to a production level of 4164 U/mL after 9611 of induction. Sorbitol co-feeding and temperature-lowering strategies both increased the beta-mannanase production levels. Combined usage of these two strategies achieved the most effective result the enzyme level reached 6336 U/mL within 84 h, which to our best knowledge is the highest production level reported for the expression of extreme beta-mannanase thus far. The strategy described in this work can also be adapted to express other important industrial enzymes with extreme properties. (C) 2011 Elsevier Inc. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]UNFOLDED PROTEIN RESPONSE ; BACILLUS SP N-16-5 ; RECOMBINANT ENZYME ; INSULIN PRECURSOR ; ALCOHOL OXIDASE ; GENE CLONING ; COPY NUMBER ; EXPRESSION ; OVEREXPRESSION ; STRAIN
收录类别SCI
语种英语
WOS记录号WOS:000295107300012
源URL[http://124.16.173.210/handle/834782/1471]  
专题天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文
作者单位1.Chinese Acad Sci, Inst Microbiol, Natl Engn Lab Ind Enzymes, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Taicheng,You, Lijin,Gong, Fuyu,et al. Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline beta-mannanase in Pichia pastoris[J]. ENZYME AND MICROBIAL TECHNOLOGY,2011,49(4):407-412.
APA Zhu, Taicheng.,You, Lijin.,Gong, Fuyu.,Xie, Minfeng.,Xue, Yanfen.,...&Ma, Yanhe.(2011).Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline beta-mannanase in Pichia pastoris.ENZYME AND MICROBIAL TECHNOLOGY,49(4),407-412.
MLA Zhu, Taicheng,et al."Combinatorial strategy of sorbitol feeding and low-temperature induction leads to high-level production of alkaline beta-mannanase in Pichia pastoris".ENZYME AND MICROBIAL TECHNOLOGY 49.4(2011):407-412.

入库方式： OAI收割

来源：天津工业生物技术研究所

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