A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp N16-5 Is Involved in Mannan Utilization
文献类型：期刊论文
| 作者 | Song, Yajian1,2; Li, Jinshan3; Meng, Shan1; Yin, Liang3; Xue, Yanfen1; Ma, Yanhe1,3,4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2016-03-15 |
| 卷号 | 11期号:3 |
| 英文摘要 | ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and galactosylmannotriose with dissociation constants in the micromolar range. Deletion of manH led to decreased growth ability of the strain when cultivated in medium with manno-oligosaccharides or mannan as the carbon source. ManH belongs to a manno-oligosaccharide transporter and plays an important role in mannan utilization by Bacillus sp. N16-5. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ABC TRANSPORT-SYSTEMS ; GEOBACILLUS-STEAROTHERMOPHILUS ; CASSETTE TRANSPORTERS ; SUBTILIS ; ETHANOL ; BIOMASS ; ENERGY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372572800020 |
| 源URL | [http://124.16.173.210/handle/834782/1528]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin Key Lab Ind Microbiol,Coll Biotechnol, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yajian,Li, Jinshan,Meng, Shan,et al. A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp N16-5 Is Involved in Mannan Utilization[J]. PLOS ONE,2016,11(3). |
| APA | Song, Yajian,Li, Jinshan,Meng, Shan,Yin, Liang,Xue, Yanfen,&Ma, Yanhe.(2016).A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp N16-5 Is Involved in Mannan Utilization.PLOS ONE,11(3). |
| MLA | Song, Yajian,et al."A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp N16-5 Is Involved in Mannan Utilization".PLOS ONE 11.3(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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