Synthetic pathway optimization for improved 1,2,4-butanetriol production
文献类型：期刊论文
| 作者 | Sun, Lei1; Yang, Fan2,3; Sun, Hongbing4; Zhu, Taicheng2; Li, Xinghua2; Li, Yin2; Xu, Zhenghong1; Zhang, Yanping2 |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 43期号:1页码:67-78 |
| 关键词 | Xylose 1,2,4-Butanetriol Escherichia coli Non-natural chemical Pathway optimization |
| 英文摘要 | 1,2,4-Butanetriol (BT) is an important non-natural chemical with a variety of industrial applications. Identifying the bottlenecks for BT biosynthesis is expected to contribute to improving the efficiency of this process. In this work, we first constructed a prototype strain for BT production by assembling a four-step synthetic pathway and disrupting the competing pathways for xylose in Escherichia coli BW25113. Using this prototype strain, we conducted systematic fine-tuning of the pathway enzyme expression level to identify the potential bottlenecks and optimize the BT biosynthesis. Production conditions were also optimized by exploring the effects of temperature, pH and cell density on BT titer. BT production was increased by 4.3-fold from the prototype strain, achieved a final titer of 1.58 g/L with a yield of 7.9 % after 72-h biotransformation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | ENGINEERED ESCHERICHIA-COLI ; GENES ; BUTANETRIOL ; PROMOTERS ; XYLOSE ; STEP ; TRP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367855800008 |
| 源URL | [http://124.16.173.210/handle/834782/1550]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Sch Pharmaceut Sci, Lab Pharmaceut Engn, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230022, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lei,Yang, Fan,Sun, Hongbing,et al. Synthetic pathway optimization for improved 1,2,4-butanetriol production[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(1):67-78. |
| APA | Sun, Lei.,Yang, Fan.,Sun, Hongbing.,Zhu, Taicheng.,Li, Xinghua.,...&Zhang, Yanping.(2016).Synthetic pathway optimization for improved 1,2,4-butanetriol production.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(1),67-78. |
| MLA | Sun, Lei,et al."Synthetic pathway optimization for improved 1,2,4-butanetriol production".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.1(2016):67-78. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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