A simple lateral flow biosensor for the rapid detection of copper(II) ions based on click chemistry
文献类型：期刊论文
| 作者 | Wang, Dou1; Ge, Chenchen2; Wang, Lin1; Xing, Xuerong3; Zeng, Lingwen1 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:92页码:75722-75727 |
| 英文摘要 | Copper(II) ions (Cu2+) at a high concentration are harmful to human health. Herein a simple and enzymefree lateral flow biosensor for the rapid detection of Cu2+ based on copper(I) ion (Cu+)-catalyzed click chemistry has been constructed for the first time. In the presence of sodium ascorbate, Cu2+ was reduced to Cu+, which could catalyze the cycloaddition between azide-DNA and alkyne/biotin-DNA in aqueous solution. The ligated DNA product could then be immobilized onto the test zone of the lateral flow biosensor to form a red band which could be unambiguously read by the naked eye. Taking advantage of the optical properties of gold nanoparticles (AuNPs) and high efficiency and selectivity of Cu+-catalyzed click chemistry, this assay enabled the visual detection of Cu2+ as low as 100 nM with excellent specificity. In comparison with conventional methods, this biosensor is more simple to operate and more cost-effective to use, and therefore has great potential in point-of-care diagnosis and environmental monitoring. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | FUNCTIONALIZED GOLD NANOPARTICLES ; ATOMIC-ABSORPTION-SPECTROMETRY ; COLORIMETRIC DETECTION ; LIGATION DNAZYME ; AQUEOUS-SOLUTION ; CU2+ DETECTION ; QUANTUM DOTS ; NANOCLUSTERS ; SENSITIVITY ; SELECTIVITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361120000085 |
| 源URL | [http://124.16.173.210/handle/834782/1570]  |
| 专题 | 天津工业生物技术研究所_酶工程实验室 马延和 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Regenerat Biol, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China 2.Guangzhou KingMed Ctr Clin Lab Co Ltd, Kingmed Diagnost Div Med Res & Dev Serv, Guangzhou 510308, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dou,Ge, Chenchen,Wang, Lin,et al. A simple lateral flow biosensor for the rapid detection of copper(II) ions based on click chemistry[J]. RSC ADVANCES,2015,5(92):75722-75727. |
| APA | Wang, Dou,Ge, Chenchen,Wang, Lin,Xing, Xuerong,&Zeng, Lingwen.(2015).A simple lateral flow biosensor for the rapid detection of copper(II) ions based on click chemistry.RSC ADVANCES,5(92),75722-75727. |
| MLA | Wang, Dou,et al."A simple lateral flow biosensor for the rapid detection of copper(II) ions based on click chemistry".RSC ADVANCES 5.92(2015):75722-75727. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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