Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile
文献类型：期刊论文
| 作者 | Li, Zhe1,2; Liu, Weidong2; Chen, Xi2; Jia, Shiru1; Wu, Qiaqiang2; Zhu, Dunming2; Ma, Yanhe2 |
| 刊名 | TETRAHEDRON
 |
| 出版日期 | 2013-04-29 |
| 卷号 | 69期号:17页码:3561-3564 |
| 关键词 | Carbonyl reductase Bioreduction Reduction of alpha-oxoaldehyde Chiral alcohol |
| 英文摘要 | The activity and enantioselectivity of a carbonyl reductase from Pichia pastoris GS115 were evaluated with a series of carbonyl compounds including aryl aldehydes, ketones, alpha- and beta-ketoesters. This recombinant enzyme possessed a broad substrate profile with the ability of reducing both aldehydes and ketones. Especially, the enzyme catalyzed the double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol with 99% yield and 99% ee by coupling with D-glucose dehydrogenase for the regeneration of cofactor NADPH, representing the first example of effective reduction of both aldehyde and ketone functional groups in one molecule by using only one enzyme. Furthermore, this study provides valuable information for guiding the future application of this versatile biocatalyst. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Organic |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ONE-POT SYNTHESIS ; ASYMMETRIC DIHYDROXYLATION ; STYRENE OXIDE ; ALCOHOL-DEHYDROGENASE ; CANDIDA-PARAPSILOSIS ; EPOXIDE HYDROLASE ; KETONE REDUCTION ; SOLANUM-TUBEROSUM ; HYDROGENATION ; OLEFINS |
| 收录类别 | SCI ; CCR |
| 语种 | 英语 |
| WOS记录号 | WOS:000316922700006 |
| 源URL | [http://124.16.173.210/handle/834782/1228]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhe,Liu, Weidong,Chen, Xi,et al. Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile[J]. TETRAHEDRON,2013,69(17):3561-3564. |
| APA | Li, Zhe.,Liu, Weidong.,Chen, Xi.,Jia, Shiru.,Wu, Qiaqiang.,...&Ma, Yanhe.(2013).Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile.TETRAHEDRON,69(17),3561-3564. |
| MLA | Li, Zhe,et al."Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile".TETRAHEDRON 69.17(2013):3561-3564. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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