Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp and Rhodococcus erythropolis
文献类型：期刊论文
| 作者 | Wang, Yu; Li, Jianjiong; Wu, Qiaqing; Zhu, Dunming |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 88页码:14-19 |
| 关键词 | Bioreduction (R)-3-Quinuclidinol Whole cell biocatalyst (S)-3-Quinuclidinol |
| 英文摘要 | Two bacterium strains, Nocardia sp. WY1202 and Rhodococcus erythropolis WY1406, were isolated from soil samples. They catalyzed the asymmetric reduction of 3-quinuclidinone to give enantiomeric pure (R)- and (S)-3-quinuclidinol, respectively. The optimal temperatures for the bioreduction by Nocardia sp. and R. erythropolis were 30 degrees C and 37 degrees C, respectively, while both strains showed highest activity at pH 8.0. Without external addition of expensive NADH or NADPH, (R)-3-quinuclidinol and (S)-3-quinuclidinol were obtained with 93% and 92% isolated yield and >99% enantiomeric excess. As such, microbial reduction by Nocardia sp. WY1202 or R. erythropolis WY1406 offers a new stereospecific approach to both antipodes of 3-quinuclidinol of pharmaceutical importance. (c) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | OPTICAL ISOMERS ; DERIVATIVES ; ANTAGONISTS ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315758400003 |
| 源URL | [http://124.16.173.210/handle/834782/1261]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Li, Jianjiong,Wu, Qiaqing,et al. Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp and Rhodococcus erythropolis[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,88:14-19. |
| APA | Wang, Yu,Li, Jianjiong,Wu, Qiaqing,&Zhu, Dunming.(2013).Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp and Rhodococcus erythropolis.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,88,14-19. |
| MLA | Wang, Yu,et al."Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp and Rhodococcus erythropolis".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 88(2013):14-19. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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