Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for D-Phenylalanine Synthesis
文献类型：期刊论文
| 作者 | Gao, Xiuzhen1,2,4; Huang, Fang3; Feng, Jinhui1,2; Chen, Xi1,2; Zhang, Hailing1,2; Wang, Zhixiang3; Wu, Qiaqing1,2; Zhu, Dunming1,2 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2013-08-01 |
| 卷号 | 79期号:16页码:5078-5081 |
| 英文摘要 | In order to enlarge the substrate binding pocket of the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, four amino acid residues (Phe146, Thr171, Arg181, and His227) were targeted for site saturation mutagenesis. Among all mutants, the single mutant H227V had a specific activity of 2.39 +/- 0.06 U . mg(-1), which was 35.1-fold enhancement over the wild-type enzyme. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | AMINO-ACID DEHYDROGENASE ; DIRECTED MUTAGENESIS ; DRUG DISCOVERY ; SUBSTRATE ; ENZYME ; PURIFICATION ; SPECIFICITY ; CREATION ; DOCKING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322161700035 |
| 源URL | [http://124.16.173.210/handle/834782/1266]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Engn Ctr Biocatalyt Technol, Tianjin, Peoples R China 3.Univ Chinese Acad Sci, Coll Chem & Chem Engn, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xiuzhen,Huang, Fang,Feng, Jinhui,et al. Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for D-Phenylalanine Synthesis[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(16):5078-5081. |
| APA | Gao, Xiuzhen.,Huang, Fang.,Feng, Jinhui.,Chen, Xi.,Zhang, Hailing.,...&Zhu, Dunming.(2013).Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for D-Phenylalanine Synthesis.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(16),5078-5081. |
| MLA | Gao, Xiuzhen,et al."Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for D-Phenylalanine Synthesis".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.16(2013):5078-5081. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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