Engineering a hydroxysteroid dehydrogenase to improve its soluble expression for the asymmetric reduction of cortisone to 11 beta-hydrocortisone
文献类型：期刊论文
| 作者 | Zhang, Dalong1,2; Zhang, Rui1,2; Zhang, Jie3; Chen, Liying3; Zhao, Chunxia3; Dong, Wenyue1,2; Zhao, Qing1,2; Wu, Qiaqing1,2; Zhu, Dunming1,2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 98期号:21页码:8879-8886 |
| 关键词 | Biocatalysis Whole-cell biotransformation Hydroxysteroid dehydrogenase 11 beta-Hydrocortisone |
| 英文摘要 | 11 beta-Hydrocortisone (11 beta-HC) is an important anti-inflammatory drug and intermediate for the synthesis of other steroids. One of the methods for the synthesis of 11 beta-HC is the asymmetric reduction of cortisone catalyzed by a highly regioselective and stereoselective 11 beta-hydroxysteroid dehydrogenase (11 beta-HSDH). However, this process has been prohibited by the poor soluble expression of the membrane-anchoring protein 11 beta-HSDH in prokaryotes. To overcome this obstacle, a mutant III-1G1 (Phe80Leu/Thr105Ser/Ala260Thr/Tyr274Stop) truncated at position 274 with improved yield of soluble protein was stepwise obtained from the 11 beta-HSDH from guinea pig by random mutagenesis combining with structural complementation assay and C-terminal truncating library screening. The improved 11 beta-HSDH mutant and glucose dehydrogenase (GDH) from Bacillus subtilis were coexpressed in Escherichia coli. The resulting whole-cell biocatalyst catalyzed the reduction of cortisone to 11 beta-HC with 98 % conversion in 20 h, laying foundation for the development of an asymmetric reduction process for the production of 11 beta-HC. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | YEAST SCHIZOSACCHAROMYCES-POMBE ; CURVULARIA-LUNATA ; ESCHERICHIA-COLI ; PROVIDE INSIGHTS ; 11-BETA-HYDROXYLATION ; HYDROCORTISONE ; TYPE-1 ; SOLUBILITY ; MONODISPERSITY ; PROTOPLASTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343904700008 |
| 源URL | [http://124.16.173.210/handle/834782/1340]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Engn Ctr Biocatalyt Technol, Tianjin 300308, Peoples R China 3.Tianjin Pharmaceut Res Inst Co Ltd, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dalong,Zhang, Rui,Zhang, Jie,et al. Engineering a hydroxysteroid dehydrogenase to improve its soluble expression for the asymmetric reduction of cortisone to 11 beta-hydrocortisone[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(21):8879-8886. |
| APA | Zhang, Dalong.,Zhang, Rui.,Zhang, Jie.,Chen, Liying.,Zhao, Chunxia.,...&Zhu, Dunming.(2014).Engineering a hydroxysteroid dehydrogenase to improve its soluble expression for the asymmetric reduction of cortisone to 11 beta-hydrocortisone.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(21),8879-8886. |
| MLA | Zhang, Dalong,et al."Engineering a hydroxysteroid dehydrogenase to improve its soluble expression for the asymmetric reduction of cortisone to 11 beta-hydrocortisone".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.21(2014):8879-8886. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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