Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase
文献类型：期刊论文
| 作者 | Liu, Weidong; Guo, Rey-Ting; Chen, Xi; Li, Zhe; Gao, Xiuzhen; Huang, Chun-Hsiang; Wu, Qiaqing; Feng, Jinhui; Zhu, Dunming |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2015-04-13 |
| 卷号 | 16期号:6页码:924-929 |
| 关键词 | amination dehydrogenases enzyme catalysis protein structures structural biology |
| 英文摘要 | A meso-diaminopimelate dehydrogenase (DAPDH) from Clostridium tetani E88 (CtDAPDH) was found to have low activity toward the D-amino acids other than its native substrate. Site-directed mutagenesis similar to that carried out on the residues mutated by Vedha-Peters et al. resulted in a mutant enzyme with highly improved catalytic ability for the synthesis of D-amino acids. The crystal structures of the CtDAPDH mutant in apo form and in complex with meso-diaminopimelate (meso-DAP), D-leucine (D-leu), and 4-methyl-2-oxopentanoic acid (MOPA) were solved. meso-DAP was found in an area outside the catalytic cavity; this suggested a possible two-step substrate-binding mechanism for meso-DAP. D-leu and MOPA each bound both to Leu154 and to Gly155 in the open form of CtDAPDH, and structural analysis revealed the molecular basis for the expanded substrate specificity of the mutant meso-diaminopimelate dehydrogenases. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | AMINO-ACID DEHYDROGENASE ; CORYNEBACTERIUM-GLUTAMICUM ; SYMBIOBACTERIUM-THERMOPHILUM ; 3-DIMENSIONAL STRUCTURE ; CRYSTALLOGRAPHY ; MICROBIOLOGY ; MUTAGENESIS ; REFINEMENT ; NUTRITION ; CREATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000352489300006 |
| 源URL | [http://124.16.173.210/handle/834782/1453]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Weidong,Guo, Rey-Ting,Chen, Xi,et al. Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase[J]. CHEMBIOCHEM,2015,16(6):924-929. |
| APA | Liu, Weidong.,Guo, Rey-Ting.,Chen, Xi.,Li, Zhe.,Gao, Xiuzhen.,...&Zhu, Dunming.(2015).Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase.CHEMBIOCHEM,16(6),924-929. |
| MLA | Liu, Weidong,et al."Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase".CHEMBIOCHEM 16.6(2015):924-929. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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