The catalytic promiscuity of a microbial 7 alpha-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds
文献类型：期刊论文
| 作者 | Liu, Yang1,2; Lv, Tong1,2; Ren, Jie1; Wang, Min2; Wu, Qiaqing1; Zhu, Dunming1 |
| 刊名 | STEROIDS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 76期号:10-11页码:1136-1140 |
| 关键词 | 7 alpha-Hydroxysteroid dehydrogenase Benzaldehydes Bioreduction Catalytic promiscuity Multi-potency |
| 英文摘要 | A thermostable 7 alpha-hydroxysteroid dehydrogenase from Bacteroides fragilis ATCC 25285 was found to catalyze the reduction of various benzaldehyde analogues to their corresponding benzyl alcohols. The enzyme activity was dependent upon the substituent on the benzene ring of the substrates. Benzaldehydes with electron-withdrawing substituent usually showed higher activity than those with electron-donating groups. Furthermore, this enzyme was tolerant to some organic solvents. These results together with previous studies suggested that 7 alpha-hydroxysteroid dehydrogenase from B. fragilis might play multiple functional roles in biosynthesis and metabolism of bile acids, and in the detoxification of xenobiotics containing carbonyl groups in the large intestine. In addition, its broad substrate spectrum offers great potential for finding applications not only in the synthesis of steroidal compounds of pharmaceutical importance, but also for the production of other high-value fine chemicals. (C) 2011 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | BACTEROIDES-FRAGILIS ; NITROSAMINE 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE ; HYDROXYSTEROID DEHYDROGENASES ; COMAMONAS TESTOSTERONI ; PARTIAL-PURIFICATION ; ACID ; CLONING ; TYPE-1 ; LIVER ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294074700024 |
| 源URL | [http://124.16.173.210/handle/834782/1474]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Coll Biotechnol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Lv, Tong,Ren, Jie,et al. The catalytic promiscuity of a microbial 7 alpha-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds[J]. STEROIDS,2011,76(10-11):1136-1140. |
| APA | Liu, Yang,Lv, Tong,Ren, Jie,Wang, Min,Wu, Qiaqing,&Zhu, Dunming.(2011).The catalytic promiscuity of a microbial 7 alpha-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds.STEROIDS,76(10-11),1136-1140. |
| MLA | Liu, Yang,et al."The catalytic promiscuity of a microbial 7 alpha-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds".STEROIDS 76.10-11(2011):1136-1140. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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