Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin
文献类型：期刊论文
| 作者 | Liu, Ningning1; Xiong, Yun2; Ren, Yiran1; Zhang, Linlin1; He, Xianfei1; Wang, Xincheng1; Liu, Min1; Li, Dengwen1; Shui, Wenqing2; Zhou, Jun1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 14期号:8页码:3292-3304 |
| 关键词 | tubulin post-translational modification microtubule assembly microtubule stability intracellular transport |
| 英文摘要 | Tubulin is known to undergo unique post-translational modifications (PTMs), such as detyrosination and polyglutamylation, particularly in the unstructured carboxy-terminal tails (CTTs). However, more conventional PTMs of tubulin and their roles in the regulation of microtubule properties and functions remain poorly defined. Here, we report the comprehensive profiling of tubulin phosphorylation, acetylation, ubiquitylation, and O-GlcNAcylation in HeLa cells with a proteomic approach. Our tubulin-targeted analysis has identified 80 residues bearing single or multiple conventional PTMs including 24 novel PTM sites not covered in previous global proteomic surveys. By using a series of PTM-deficient or PTM-mimicking mutants, we further find that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. In addition, these tubulin PTMs have distinct effects on the retrograde transport of adenoviruses along microtubules. These findings thus enlarge the repertoire of tubulin PTMs and foster our understanding of their versatile roles in the regulation of microtubule dynamics and cellular functions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | BOVINE IMMUNODEFICIENCY VIRUS ; CHLAMYDOMONAS ALPHA-TUBULIN ; LYSINE ACETYLATION ; TYROSINE KINASE ; BETA-TUBULIN ; CELL-CYCLE ; MICROTUBULES ; TRANSPORT ; PROTEIN ; SITES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000359394000024 |
| 源URL | [http://124.16.173.210/handle/834782/1512]  |
| 专题 | 天津工业生物技术研究所_生物质谱与定量生物学研究 水雯箐_期刊论文 |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ningning,Xiong, Yun,Ren, Yiran,et al. Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(8):3292-3304. |
| APA | Liu, Ningning.,Xiong, Yun.,Ren, Yiran.,Zhang, Linlin.,He, Xianfei.,...&Zhou, Jun.(2015).Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin.JOURNAL OF PROTEOME RESEARCH,14(8),3292-3304. |
| MLA | Liu, Ningning,et al."Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin".JOURNAL OF PROTEOME RESEARCH 14.8(2015):3292-3304. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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