Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis
文献类型：期刊论文
| 作者 | He, Xianfei1; Liu, Zhu2; He, Qianqian1; Qin, Juan1; Liu, Ningning1; Zhang, Linlin1; Li, Dengwen1; Zhou, Jun1; Shui, Wenqing1,3; Liu, Min2 |
| 刊名 | THORACIC CANCER
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 6期号:5页码:649-654 |
| 关键词 | Mass spectrometry microtubule microtubule-binding protein taxol tubulin |
| 英文摘要 | Microtubule-binding proteins (MBPs) are structurally and functionally diverse regulators of microtubule-mediated cellular processes. Alteration of MBPs has been implicated in the pathogenesis of human diseases, including cancer. MBPs can stabilize or destabilize microtubules or move along microtubules to transport various cargoes. In addition, MBPs can control microtubule dynamics through direct interaction with microtubules or coordination with other proteins. To better understand microtubule structure and function, it is necessary to identify additional MBPs. In this study, we isolated microtubules and MBPs from mammalian cells by a taxol-based method and then profiled a panel of MBPs by mass spectrometry. We discovered a number of previously uncharacterized MBPs, including several membrane-associated proteins and proteins involved in post-translational modifications, in addition to several structural components. These results support the notion that microtubules have a wide range of functions and may undergo more exquisite regulation than previously recognized. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Oncology ; Respiratory System |
| 研究领域[WOS] | Oncology ; Respiratory System |
| 关键词[WOS] | TUMOR-SUPPRESSOR CYLD ; PACLITAXEL SENSITIVITY ; CELL-MIGRATION ; BREAST-CANCER ; POSTTRANSLATIONAL MODIFICATIONS ; DEPENDENT MECHANISM ; AURORA-B ; TUBULIN ; EB1 ; CEP70 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000360746400014 |
| 源URL | [http://124.16.173.210/handle/834782/1522]  |
| 专题 | 天津工业生物技术研究所_生物质谱与定量生物学研究 水雯箐_期刊论文 |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Tianjin Med Univ, Dept Biochem, Sch Basic Med Sci, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xianfei,Liu, Zhu,He, Qianqian,et al. Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis[J]. THORACIC CANCER,2015,6(5):649-654. |
| APA | He, Xianfei.,Liu, Zhu.,He, Qianqian.,Qin, Juan.,Liu, Ningning.,...&Liu, Min.(2015).Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis.THORACIC CANCER,6(5),649-654. |
| MLA | He, Xianfei,et al."Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis".THORACIC CANCER 6.5(2015):649-654. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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