New HDAC6-mediated deacetylation sites of tubulin in the mouse brain identified by quantitative mass spectrometry
文献类型：期刊论文
| 作者 | Liu, Ningning1; Xiong, Yun2; Li, Shanshan2; Ren, Yiran1; He, Qianqian1; Gao, Siqi1; Zhou, Jun1; Shui, Wenqing2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-11-19 |
| 卷号 | 5 |
| 英文摘要 | The post-translational modifications (PTMs) occurring on microtubules have been implicated in the regulation of microtubule properties and functions. Acetylated K40 of alpha-tubulin, a hallmark of long-lived stable microtubules, is known to be negatively controlled by histone deacetylase 6 (HDAC6). However, the vital roles of HDAC6 in microtubule-related processes such as cell motility and cell division cannot be fully explained by the only known target site on tubulin. Here, we attempt to comprehensively map lysine acetylation sites on tubulin purified from mouse brain tissues. Furthermore, mass spectrometry-based quantitative comparison of acetylated peptides from wildtype vs HDAC6 knockout mice allowed us to identify six new deacetylation sites possibly mediated by HDAC6. Thus, adding new sites to the repertoire of HDAC6-mediated tubulin deacetylation events would further our understanding of the multi-faceted roles of HDAC6 in regulating microtubule stability and cellular functions. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CHLAMYDOMONAS ALPHA-TUBULIN ; HISTONE H3 ; IN-VIVO ; HDAC6 ; ACETYLATION ; MICROTUBULES ; MICE ; ACETYLTRANSFERASE ; INHIBITORS ; DISEASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364933800001 |
| 源URL | [http://124.16.173.210/handle/834782/1559]  |
| 专题 | 天津工业生物技术研究所_生物质谱与定量生物学研究 水雯箐_期刊论文 |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ningning,Xiong, Yun,Li, Shanshan,et al. New HDAC6-mediated deacetylation sites of tubulin in the mouse brain identified by quantitative mass spectrometry[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Liu, Ningning.,Xiong, Yun.,Li, Shanshan.,Ren, Yiran.,He, Qianqian.,...&Shui, Wenqing.(2015).New HDAC6-mediated deacetylation sites of tubulin in the mouse brain identified by quantitative mass spectrometry.SCIENTIFIC REPORTS,5. |
| MLA | Liu, Ningning,et al."New HDAC6-mediated deacetylation sites of tubulin in the mouse brain identified by quantitative mass spectrometry".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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