Production of salidroside in metabolically engineered Escherichia coli
文献类型：期刊论文
| 作者 | Bai, Yanfen1,2,3; Bi, Huiping1,2; Zhuang, Yibin1,2; Liu, Chang1,2,3; Cai, Tao1; Liu, Xiaonan1,2,3; Zhang, Xueli1,2; Liu, Tao1,2; Ma, Yanhe1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-10-17 |
| 卷号 | 4 |
| 英文摘要 | Salidroside (1) is the most important bioactive component of Rhodiola (also called as "Tibetan Ginseng''), which is a valuable medicinal herb exhibiting several adaptogenic properties. Due to the inefficiency of plant extraction and chemical synthesis, the supply of salidroside (1) is currently limited. Herein, we achieved unprecedented biosynthesis of salidroside (1) from glucose in a microorganism. First, the pyruvate decarboxylase ARO10 and endogenous alcohol dehydrogenases were recruited to convert 4-hydroxyphenylpyruvate (2), an intermediate of L-tyrosine pathway, to tyrosol (3) in Escherichia coli. Subsequently, tyrosol production was improved by overexpressing the pathway genes, and by eliminating competing pathways and feedback inhibition. Finally, by introducing Rhodiola-derived glycosyltransferase UGT73B6 into the above-mentioned recombinant strain, salidroside (1) was produced with a titer of 56.9 mg/L. Interestingly, the Rhodiola-derived glycosyltransferase, UGT73B6, also catalyzed the attachment of glucose to the phenol position of tyrosol (3) to form icariside D2 (4), which was not reported in any previous literatures. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | L-TYROSINE PRODUCTION ; RHODIOLA-SACHALINENSIS ; SACCHAROMYCES-CEREVISIAE ; MICROBIAL-PRODUCTION ; MOLECULAR-CLONING ; SALICYLIC-ACID ; CAFFEIC ACID ; PATHWAY ; BIOSYNTHESIS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343293800001 |
| 源URL | [http://124.16.173.210/handle/834782/1407]  |
| 专题 | 天津工业生物技术研究所_天然化合物合成生物学实验室 刘涛_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yanfen,Bi, Huiping,Zhuang, Yibin,et al. Production of salidroside in metabolically engineered Escherichia coli[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Bai, Yanfen.,Bi, Huiping.,Zhuang, Yibin.,Liu, Chang.,Cai, Tao.,...&Ma, Yanhe.(2014).Production of salidroside in metabolically engineered Escherichia coli.SCIENTIFIC REPORTS,4. |
| MLA | Bai, Yanfen,et al."Production of salidroside in metabolically engineered Escherichia coli".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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