Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement
文献类型：期刊论文
| 作者 | Yin, Hua; Zhuang, Yi-bin; Li, E-e; Bi, Hui-ping; Zhou, Wei; Liu, Tao |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2015-06-01 |
| 卷号 | 37期号:6页码:1249-1255 |
| 关键词 | Costunolide E. coli Farnesyl diphosphate Heterologous biosynthesis Mevalonate pathway Radix aucklandiae Sesquiterpene lactones (SLs) |
| 英文摘要 | Costunolide, the main bioactive compound of the medicinal plant, Radix Aucklandiae, is a sesquiterpene lactone (SL) and has a broad range of biological activities. It is also a precursor of many biologically-active SLs and is a branching point in the biosynthesis of SLs. Here we have reconstituted the costunolide biosynthetic pathway in Escherichia coli by co-expression of three genes (GAS, GAO, LsCOS) involved in costunolide biosynthesis and eight genes involved in converting acetyl-CoA into farnesyl diphosphate from mevalonate pathway. Costunolide production was then detected. By screening and optimization of cultured medium and inducing temperature, costunolide yield was up to 100 mg l(-1) in E. coli. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SESQUITERPENE LACTONES ; IDENTIFICATION ; PARTHENOLIDE ; EXPRESSION ; CHICORY ; CELLS ; PLANT ; L. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000354498900015 |
| 源URL | [http://124.16.173.210/handle/834782/1462]  |
| 专题 | 天津工业生物技术研究所_天然化合物合成生物学实验室 刘涛_期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Hua,Zhuang, Yi-bin,Li, E-e,et al. Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement[J]. BIOTECHNOLOGY LETTERS,2015,37(6):1249-1255. |
| APA | Yin, Hua,Zhuang, Yi-bin,Li, E-e,Bi, Hui-ping,Zhou, Wei,&Liu, Tao.(2015).Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement.BIOTECHNOLOGY LETTERS,37(6),1249-1255. |
| MLA | Yin, Hua,et al."Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement".BIOTECHNOLOGY LETTERS 37.6(2015):1249-1255. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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