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Engineered short branched-chain acyl-CoA synthesis in E-coli and acylation of chloramphenicol to branched-chain derivatives

文献类型：期刊论文

作者Bi, Huiping1,2; Bai, Yanfen1,2; Cai, Tao1,3; Zhuang, Yibin1,2; Liang, Xiaomei1,2; Zhang, Xueli1,2; Liu, Tao1,2; Ma, Yanhe1,3
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2013-12-01
卷号97期号:24页码:10339-10348
关键词Branched-chain acyl-CoAs BCDH complex Heterologous biosynthesis Chloramphenicol derivatives E. coli
英文摘要Short branched-chain acyl-CoAs are important building blocks for a wide variety of pharmaceutically valuable natural products. Escherichia coli has been used as a heterologous host for the production of a variety of natural compounds for many years. In the current study, we engineered synthesis of isobutyryl-CoA and isovaleryl-CoA from glucose in E. coli by integration of the branched-chain alpha-keto acid dehydrogenase complex from Streptomyces avermitilis. In the presence of the chloramphenicol acetyltransferase (cat) gene, chloramphenicol was converted to both chloramphenicol-3-isobutyrate and chloramphenicol-3-isovalerate by the recombinant E. coli strains, which suggested successful synthesis of isobutyryl-CoA and isovaleryl-CoA. Furthermore, we improved the alpha-keto acid precursor supply by overexpressing the alsS gene from Bacillus subtilis and the ilvC and ilvD genes from E. coli and thus enhanced the synthesis of short branched-chain acyl-CoAs. By feeding 25 mg/L chloramphenicol, 2.96 +/- 0.06 mg/L chloramphenicol-3-isobutyrate and 3.94 +/- 0.06 mg/L chloramphenicol-3-isovalerate were generated by the engineered E. coli strain, which indicated efficient biosynthesis of short branched-chain acyl-CoAs. HPLC analysis showed that the most efficient E. coli strain produced 80.77 +/- 3.83 nmol/g wet weight isovaleryl-CoA. To our knowledge, this is the first report of production of short branched-chain acyl-CoAs in E. coli and opens a way to biosynthesize various valuable natural compounds based on these special building blocks from renewable carbon sources.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]KETO ACID DEHYDROGENASE ; GENE-CLUSTER ; STREPTOMYCES-AVERMITILIS ; AVERMECTIN BIOSYNTHESIS ; PYRUVATE-DEHYDROGENASE ; BACILLUS-SUBTILIS ; SEQUENCE-ANALYSIS ; ANTIBIOTICS ; LIPOMYCIN ; COMPONENT
收录类别SCI
语种英语
WOS记录号WOS:000327495000009
源URL[http://124.16.173.210/handle/834782/1236]  
专题天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文
作者单位1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
3.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式
GB/T 7714		 Bi, Huiping,Bai, Yanfen,Cai, Tao,et al. Engineered short branched-chain acyl-CoA synthesis in E-coli and acylation of chloramphenicol to branched-chain derivatives[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(24):10339-10348.
APA Bi, Huiping.,Bai, Yanfen.,Cai, Tao.,Zhuang, Yibin.,Liang, Xiaomei.,...&Ma, Yanhe.(2013).Engineered short branched-chain acyl-CoA synthesis in E-coli and acylation of chloramphenicol to branched-chain derivatives.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(24),10339-10348.
MLA Bi, Huiping,et al."Engineered short branched-chain acyl-CoA synthesis in E-coli and acylation of chloramphenicol to branched-chain derivatives".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.24(2013):10339-10348.

入库方式： OAI收割

来源：天津工业生物技术研究所

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