Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides
文献类型：期刊论文
| 作者 | Dai, Zhubo1,2; Liu, Yi1,2,3; Zhang, Xianan4,5; Shi, Mingyu1,2,3; Wang, Beibei1,2,3; Wang, Dong1,2,3; Huang, Luqi4; Zhang, Xueli1,2 |
| 刊名 | METABOLIC ENGINEERING
 |
| 出版日期 | 2013-11-01 |
| 卷号 | 20页码:146-156 |
| 关键词 | Ginsenosides Protopanaxadiol Dammarenediol-II Metabolic engineering Saccharomyces cerevisiae |
| 英文摘要 | Ginsenosides are the primary bioactive components of ginseng, which is a popular medicinal herb and exhibits diverse pharmacological activities. Protopanaxadiol is the aglycon of several dammarane-type ginsenosides, which also has anticancer activity. For microbial production of protopanaxadiol, dammarenediol-II synthase and protopanaxadiol synthase genes of Panax ginseng, together with a NADPH-cytochrome P450 reductase gene of Arubidopsis thaliana, were introduced into Saccharomyces cerevisiae, resulting in production of 0.05 mg/g DCW protopanaxadiol. Increasing squalene and 2,3-oxidosqualene supplies through overexpressing truncated 3-hydroxyl-3-methylglutaryl-CoA reductase, farnesyl diphosphate synthase, squalene synthase and 2,3-oxidosqualene synthase genes, together with increasing protopanaxadiol synthase activity through codon optimization, led to 262 fold increase of protopanaxadiol production. Finally, using two phase extractive fermentation resulted in production of 8.40 mg/g DCW protopanaxadiol (1189 mg/L), together with 10.94 mg/g DCW dammarenediol-II (1548 mg/L). The yeast strains engineered in this work can serve as the basis for creating an alternative way for production of ginsenosides in place of extraction from plant sources. (C) 2013 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | HIGH-LEVEL PRODUCTION ; COENZYME-A REDUCTASE ; PANAX-GINSENG ; SQUALENE SYNTHASE ; BIOSYNTHETIC-PATHWAY ; MEVALONATE PATHWAY ; ESCHERICHIA-COLI ; ISOPRENOID PATHWAY ; ARTEMISINIC ACID ; DAMMARENEDIOL-II |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328080100016 |
| 源URL | [http://124.16.173.210/handle/834782/1271]  |
| 专题 | 天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Beijing 100864, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China 4.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China 5.Capital Med Univ, Sch Tradit Chinese Med, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Zhubo,Liu, Yi,Zhang, Xianan,et al. Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides[J]. METABOLIC ENGINEERING,2013,20:146-156. |
| APA | Dai, Zhubo.,Liu, Yi.,Zhang, Xianan.,Shi, Mingyu.,Wang, Beibei.,...&Zhang, Xueli.(2013).Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides.METABOLIC ENGINEERING,20,146-156. |
| MLA | Dai, Zhubo,et al."Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides".METABOLIC ENGINEERING 20(2013):146-156. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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