Producing aglycons of ginsenosides in bakers' yeast
文献类型：期刊论文
| 作者 | Dai, Zhubo1,2; Wang, Beibei1,2,3; Liu, Yi1,2,3; Shi, Mingyu1,2,3; Wang, Dong1,2,3; Zhang, Xianan4,5; Liu, Tao1,2; Huang, Luqi4; Zhang, Xueli1,2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-01-15 |
| 卷号 | 4 |
| 英文摘要 | Ginsenosides are the primary bioactive components of ginseng, which is a popular medicinal plant that exhibits diverse pharmacological activities. Protopanaxadiol, protopanaxatriol and oleanolic acid are three basic aglycons of ginsenosides. Producing aglycons of ginsenosides in Saccharomyces cerevisiae was realized in this work and provides an alternative route compared to traditional extraction methods. Synthetic pathways of these three aglycons were constructed in S. cerevisiae by introducing beta-amyrin synthase, oleanolic acid synthase, dammarenediol-II synthase, protopanaxadiol synthase, protopanaxatriol synthase and NADPH-cytochrome P450 reductase from different plants. In addition, a truncated 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthase and 2,3-oxidosqualene synthase genes were overexpressed to increase the precursor supply for improving aglycon production. Strain GY-1 was obtained, which produced 17.2 mg/L protopanaxadiol, 15.9 mg/L protopanaxatriol and 21.4 mg/L oleanolic acid. The yeast strains engineered in this work can serve as the basis for creating an alternative way for producing ginsenosides in place of extractions from plant sources. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SQUALENE SYNTHASE GENE ; SACCHAROMYCES-CEREVISIAE ; PANAX-GINSENG ; TRANSCRIPTIONAL REGULATION ; MEVALONATE PATHWAY ; DAMMARENEDIOL-II ; BIOSYNTHESIS ; TRITERPENE ; CYTOCHROME-P450 ; OVEREXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329847100006 |
| 源URL | [http://124.16.173.210/handle/834782/1347]  |
| 专题 | 天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Beijing 100864, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Beijing 100864, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China 4.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China 5.Capital Med Univ, Sch Tradit Chinese Med, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Zhubo,Wang, Beibei,Liu, Yi,et al. Producing aglycons of ginsenosides in bakers' yeast[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Dai, Zhubo.,Wang, Beibei.,Liu, Yi.,Shi, Mingyu.,Wang, Dong.,...&Zhang, Xueli.(2014).Producing aglycons of ginsenosides in bakers' yeast.SCIENTIFIC REPORTS,4. |
| MLA | Dai, Zhubo,et al."Producing aglycons of ginsenosides in bakers' yeast".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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