Production of lycopene by metabolically-engineered Escherichia coli
文献类型：期刊论文
| 作者 | Sun, Tao1,2,3; Miao, Liangtian1,2,3; Li, Qingyan2,3; Dai, Guanping1,2,3; Lu, Fuping1; Liu, Tao2,3; Zhang, Xueli2,3; Ma, Yanhe2 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 36期号:7页码:1515-1522 |
| 关键词 | beta-Carotene Escherichia coli Isoprenoid biosynthesis Lycopene NADPH Ribosome-binding sites |
| 英文摘要 | Escherichia coli strain CAR001 that produces beta-carotene was genetically engineered to produce lycopene by deleting genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the crtEXYIB operon. The resulting strain, LYC001, produced 10.5 mg lycopene/l (6.5 mg/g dry cell weight, DCW). Modulating expression of genes encoding alpha-ketoglutarate dehydrogenase, succinate dehydrogenase and transaldolase B within central metabolic modules increased NADPH and ATP supplies, leading to a 76 % increase of lycopene yield. Ribosome binding site libraries were further used to modulate expression of genes encoding 1-deoxy-d-xylulose-5-phosphate synthase (dxs) and isopentenyl diphosphate isomerase (idi) and the crt gene operon, which improved the lycopene yield by 32 %. The optimal strain LYC010 produced 3.52 g lycopene/l (50.6 mg/g DCW) in fed-batch fermentation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | BETA-CAROTENE ; E. COLI ; GENES ; BIOSYNTHESIS ; MEVALONATE ; STRAINS ; PATHWAY ; DISEASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337139500017 |
| 源URL | [http://124.16.173.210/handle/834782/1391]  |
| 专题 | 天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Tao,Miao, Liangtian,Li, Qingyan,et al. Production of lycopene by metabolically-engineered Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2014,36(7):1515-1522. |
| APA | Sun, Tao.,Miao, Liangtian.,Li, Qingyan.,Dai, Guanping.,Lu, Fuping.,...&Ma, Yanhe.(2014).Production of lycopene by metabolically-engineered Escherichia coli.BIOTECHNOLOGY LETTERS,36(7),1515-1522. |
| MLA | Sun, Tao,et al."Production of lycopene by metabolically-engineered Escherichia coli".BIOTECHNOLOGY LETTERS 36.7(2014):1515-1522. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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